Vinyl-aryl derivatives for inflammation and immune-related uses

ABSTRACT

The invention relates to compounds that are useful as immunosuppressive agents and for treating and preventing inflammatory conditions, allergic disorders, and immune disorders.

RELATED APPLICATIONS

This application claims the benefit of U.S. Provisional Application No. 60/962,823, filed Aug. 1, 2007, the entire teachings of which are incorporated herein by reference.

FIELD OF THE INVENTION

This invention relates to biologically active chemical compounds, namely vinyl-phenyl derivatives that may be used for immunosuppression or to treat or prevent inflammatory conditions, allergic disorders and immune disorders.

BACKGROUND OF THE INVENTION

Inflammation is a mechanism that protects mammals from invading pathogens. However, while transient inflammation is necessary to protect a mammal from infection, uncontrolled inflammation causes tissue damage and is the underlying cause of many illnesses. Inflammation is typically initiated by binding of an antigen to T-cell antigen receptor. Antigen binding by a T-cell initiates calcium influx into the cell via calcium ion channels, such as Ca²⁺-release-activated Ca²⁺ channels (CRAC). Calcium ion influx in turn initiates a signaling cascade that leads to activation of these cells and an inflammatory response characterized by cytokine production.

Interleukin 2 (IL-2) is a cytokine that is secreted by T cells in response to calcium ion influx into the cell. IL-2 modulates immunological effects on many cells of the immune system. For example, it is a potent T cell mitogen that is required for T cell proliferation, promoting their progression from G1 to S phase of the cell cycle; it stimulates the growth of NK cells; and it acts as a growth factor to B cells and stimulates antibody synthesis.

IL-2, although useful in the immune response, can cause a variety of problems. IL-2 damages the blood-brain barrier and the endothelium of brain vessels. These effects may be the underlying causes of neuropsychiatric side effects observed under IL-2 therapy, e.g. fatigue, disorientation and depression. It also alters the electrophysiological behavior of neurons.

Due to its effects on both T and B cells, IL-2 is a major central regulator of immune responses. It plays a role in inflammatory reactions, tumor surveillance, and hematopoiesis.

It also affects the production of other cytokines, inducing IL-1, TNF-α and TNF-β secretion, as well as stimulating the synthesis of IFN-γ in peripheral leukocytes.

T cells that are unable to produce IL-2 become inactive (anergic). This renders them potentially inert to any antigenic stimulation they might receive in the future. As a result, agents which inhibit IL-2 production can be used for immunosuppression or to treat or prevent inflammation and immune disorders. This approach has been clinically validated with immunosuppressive drugs such as cyclosporin, FK506, and RS61443. Despite this proof of concept, agents that inhibit IL-2 production remain far from ideal. Among other problems, efficacy limitations and unwanted side effects (including dose-dependant nephrotoxicity and hypertension) hinder their use.

Over production of proinflammatory cytokines other than IL-2 has also been implicated in many autoimmune diseases. For example, Interleukin 5 (IL-5), a cytokine that increases the production of eosinophils, is increased in asthma. Overproduction of IL-5 is associated with accumulation of eosinophils in the asthmatic bronchial mucosa, a hall mark of allergic inflammation. Thus, patients with asthma and other inflammatory disorders involving the accumulation of eosinophils would benefit from the development of new drugs that inhibit the production of IL-5.

Interleukin 4 (IL-4) and interleukin 13 (IL-13) have been identified as mediators of the hypercontractility of smooth muscle found in inflammatory bowel disease and asthma. Thus, patients with asthma and inflammatory bowel disease would benefit from the development of new drugs that inhibit IL-4 and IL-13 production.

Granulocyte macrophage-colony stimulating factor (GM-CSF) is a regulator of maturation of granulocyte and macrophage lineage population and has been implicated as a key factor in inflammatory and autoimmune diseases. Anti-GM-CSF antibody blockade has been shown to ameliorate autoimmune disease. Thus, development of new drugs that inhibit the production of GM-CSF would be beneficial to patients with an inflammatory or autoimmune disease.

There is a continuing need for new drugs which overcome one or more of the shortcomings of drugs currently used for immunosuppression or in the treatment or prevention of inflammatory disorders, allergic disorders and autoimmune disorders. Desirable properties of new drugs include efficacy against diseases or disorders that are currently untreatable or poorly treatable, new mechanism of action, oral bioavailability and/or reduced side effects.

SUMMARY OF THE INVENTION

This invention meets the above-mentioned needs by providing certain vinyl-phenyl derivatives that inhibit the activity of CRAC ion channels and inhibit the production of IL-2, IL-4, IL-5, IL-13, GM-CSF, TNF-α, and IFNγ. These compounds are particularly useful for immunosuppression and/or to treat or prevent inflammatory conditions, allergic disorders and immune disorders.

In one embodiment, the invention relates to compounds of structural formula (I):

-   -   or a pharmaceutically acceptable salt, solvate, clathrate, or         prodrug thereof, wherein:     -   X₁ and X₂ are, independently, CH or N;     -   one of X₃ or X₆ is NR₂₉, and the other is CH₂, CHR₂, or C(R₂)₂;     -   L is a linker selected from the group consisting of —NHCH₂—,         —CH₂NH—, —NR₅—C(O)—, —C(O)—NR₅—, —NR₅—C(S)—, —C(S)—NR₅—,         —NR₅—C(NR₈)—, —C(NR₈)—NR₅—, —NR₅C(S)NR₅—, —NR₅C(NR₈)NR₅—, or         —NR₅CR^(a)R^(b)NR₅—;     -   Y is a substituted phenyl, provided that Y is not substituted         with —NHC(O)R₃₀;     -   R₁ is H or a substituent;     -   R₂, for each occurrence is, independently, a substituent,         provided that R₂ is not ═O or ═C(R₂₈)₂;     -   R₄ is a substituent;     -   R^(a) and R^(b), for each occurrence, are independently, H, an         optionally substituted alkyl, an optionally substituted alkenyl,         an optionally substituted alkynyl, an optionally substituted         cycloalkyl, an optionally substituted cycloalkenyl, an         optionally substituted heterocyclyl, an optionally substituted         aryl, an optionally substituted heteroaryl, an optionally         substituted aralkyl, an optionally substituted heteraralkyl,         cyano, nitro, halo, —OR₅, —SR₅, —NR₆R₇, —C(O)NR₆R₇, —NR₅C(O)R₅,         —C(O)R₅, —C(O)OR₅, —OC(O)R₅, —C(O)SR₅, —SC(O)R₅, —C(S)NR₆R₇,         —NR₅C(S)R₅, —C(S)R₅, —C(S)OR₅, —OC(S)R₅, —C(S)SR₅, —SC(S)R₅,         —C(NR₈)NR₆R₇, —NR₅C(NR₈)R₅, —C(NR₈)R₅, —C(NR₈)OR₅, —OC(NR₈)R₅,         —C(NR₈)SR₅, —SC(NR₈)R₅, —OC(O)OR₅, —OC(O)NR₆R₇, —NR₅C(O)OR₅,         —NR₅C(O)NR₆R₇, —SC(O)OR₅, —SC(O)NR₆R₇, —SC(O)SR₅, —NR₅C(O)SR₅,         —OC(O)SR₅, —OC(S)OR₅, —OC(S)NR₆R₇, —NR₅C(S)OR₅, —NR₅C(S)NR₆R₇,         —SC(S)OR₅, —SC(S)NR₆R₇, —SC(S)SR₅, —NR₅C(S)SR₅, —OC(S)SR₅,         —OC(NR₈)OR₅, —OC(NR₈)NR₆R₇, —NR₅C(NR₈)OR₅, —NR₅C(NR₈)NR₆R₇,         —SC(NR₈)OR₅, —SC(NR₈)NR₆R₇, —SC(NR₈)SR₅, —NR₅C(NR₈)SR₅, or         —OC(NR₈)SR₅;     -   R₅, for each occurrence, is independently, H, an optionally         substituted alkyl, an optionally substituted alkenyl, an         optionally substituted alkynyl, an optionally substituted         cycloalkyl, an optionally substituted cycloalkenyl, an         optionally substituted heterocyclyl, an optionally substituted         aryl, an optionally substituted heteroaryl, an optionally         substituted aralkyl, or an optionally substituted heteraralkyl;     -   R₆ and R₇, for each occurrence are, independently, H, an         optionally substituted alkyl, an optionally substituted alkenyl,         an optionally substituted alkynyl, an optionally substituted         cycloalkyl, an optionally substituted cycloalkenyl, an         optionally substituted heterocyclyl, an optionally substituted         aryl, an optionally substituted heteroaryl, an optionally         substituted aralkyl, or an optionally substituted heteraralkyl;         or R₆ and R₇ taken together with the nitrogen to which they are         attached are an optionally substituted heterocyclyl or         optionally substituted heteroaryl;     -   R₈, for each occurrence, is independently —H, a halo, an

alkyl, —OR₅, —NR₆R₇, —C(O)R₅, —C(O)OR₅, or —C(O)NR₆R₇;

-   -   R₂₈, for each occurrence is, independently, H or a substituent;     -   R₂₉ is —C(O)R₃₀ or a N-containing 6-membered aromatic ring;     -   R₃₀ is —H, —OR₅, —SR₅, —NR₆R₇, an optionally substituted alkyl,         an optionally substituted alkenyl, an optionally substituted         alkynyl, an optionally substituted cycloalkyl, an optionally         substituted cycloalkenyl, an optionally substituted         heterocyclyl, an optionally substituted aryl, an optionally         substituted heteroaryl, an optionally substituted aralkyl, or an         optionally substituted heteraralkyl;     -   m is 0, 1, or 2; and     -   n is 0, 1 or 2.

In one aspect, the compound of formula (I) is not:

-   5-[4-(2,6-difluoro-benzoylamino)-phenyl]-4-methyl-2,4-dihydro-2H-pyridine-1-carboxylic     acid ethyl ester; -   Ethyl     3-(4-(2,6-difluorobenzamido)phenyl)-4-methyl-5,6-dihydropyridine-1(2H)-carboxylate; -   Ethyl     4-(4-(2,6-difluorobenzamido)phenyl)-3-methyl-5,6-dihydropyridine-1     (2H)-carboxylate; -   2,6-Difluoro-N-[4-(1-methoxycarbonyl-4-thiazol-2-yl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-[4-(1-methoxycarbonyl-4-isopropyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-[4-(1-methoxycarbonyl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-[4-(1-allyloxycarbonyl-3-methyl-1,2,5,6-tetrahydro-pyridin-4-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-[4-(1-phenyloxycarbonyl-3-methyl-1,2,5,6-tetrahydro-pyridin-4-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-[4-(1-acetyl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-[4-(1-cyclopropylcarbonyl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-{4-[1-(pyridine-3-carbonyl)-cyclopropanecarbonyl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl]-phenyl}-benzamide;     or -   2,6-Difluoro-N-[4-(1-pyrimidin-2-yl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide.

A compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof is particularly useful inhibiting immune cell (e.g., mast cells, T-cells and/or B-cells) activation (e.g., cytokine production and/or proliferation in response to an antigen; and or mast cell degranulation). In particular, a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof can inhibit the production of certain cytokines that regulate immune cell activation. For example, a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof can inhibit the production of IL-2, IL-4, IL-5, IL-13, GM-CSF, TNF-α, INF-γ or combinations thereof. Moreover, a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof can modulate the activity of one or more ion channel involved in activation of immune cells, such as CRAC ion channels.

In one embodiment, compounds of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof are particularly useful for inhibiting mast cell degranulation. Mast cell degranulation has been implicated in allergic reactions.

A compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof is particularly useful for immunosuppression or for treating or preventing inflammatory conditions, allergic disorders, and immune disorders.

The invention also encompasses pharmaceutical compositions comprising a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof; and a pharmaceutically acceptable carrier or vehicle. These compositions may further comprise additional agents. These compositions are useful for immunosuppression and treating or preventing inflammatory conditions, allergic disorders and immune disorders.

The invention further encompasses methods for treating or preventing inflammatory conditions, allergic disorders, and immune disorders, comprising administering to a subject in need thereof an effective amount of a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof, or a pharmaceutical composition comprising a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof. These methods may also comprise administering to the subject an additional agent separately or in a combination composition with the compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof.

The invention further encompasses methods for suppressing the immune system of a subject, comprising administering to a subject in need thereof an effective amount of a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof, or a pharmaceutical composition comprising a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof. These methods may also comprise administering to the subject an additional agent separately or in a combination composition with the compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof.

The invention further encompasses methods for inhibiting immune cell activation, including inhibiting proliferation of T cells and/or B cells, in vivo or in vitro comprising administering to the cell an effective amount of a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof or a pharmaceutical composition comprising a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof.

The invention further encompasses methods for inhibiting cytokine production in a cell (e.g., IL-2, IL-4, IL-5, IL-13, GM-CSF, TNF-α, and/or INF-γ production) in vivo or in vitro comprising administering to a cell an effective amount of a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof or a pharmaceutical composition comprising a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof.

The invention further encompasses methods for modulating ion channel activity (e.g., CRAC channel activity) in vivo or in vitro comprising administering an effective amount of a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof or a pharmaceutical composition comprising a compound of the invention or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof.

All of the methods of this invention may be practice with a compound of the invention alone, or in combination with other agents, such as other immunosuppressive agents, anti-inflammatory agents, agents for the treatment of allergic disorders or agents for the treatment of immune disorders.

DETAILED DESCRIPTION OF THE INVENTION Definitions

Unless otherwise specified, the below terms used herein are defined as follows:

As used herein, the term an “aromatic ring” or “aryl” means a monocyclic or polycyclic-aromatic ring or ring radical comprising carbon and hydrogen atoms. Examples of suitable aryl groups include, but are not limited to, phenyl, tolyl, anthacenyl, fluorenyl, indenyl, azulenyl, and naphthyl, as well as benzo-fused carbocyclic moieties such as 5,6,7,8-tetrahydronaphthyl. An aryl group can be unsubstituted or substituted with one or more substituents (including without limitation alkyl (preferably, lower alkyl or alkyl substituted with one or more halo), hydroxy, alkoxy (preferably, lower alkoxy), alkylsulfanyl, cyano, halo, amino, and nitro. In certain embodiments, the aryl group is a monocyclic ring, wherein the ring comprises 6 carbon atoms.

As used herein, the term “alkyl” means a saturated straight chain or branched non-cyclic hydrocarbon typically having from 1 to 10 carbon atoms. Representative saturated straight chain alkyls include methyl, ethyl, n-propyl, n-butyl, n-pentyl, n-hexyl, n-heptyl, n-octyl, n-nonyl and n-decyl; while saturated branched alkyls include isopropyl, sec-butyl, isobutyl, tert-butyl, isopentyl, 2-methylbutyl, 3-methylbutyl, 2-methylpentyl, 3-methylpentyl, 4-methylpentyl, 2-methylhexyl, 3-methylhexyl, 4-methylhexyl, 5-methylhexyl, 2,3-dimethylbutyl, 2,3-dimethylpentyl, 2,4-dimethylpentyl, 2,3-dimethylhexyl, 2,4-dimethylhexyl, 2,5-dimethylhexyl, 2,2-dimethylpentyl, 2,2-dimethylhexyl, 3,3-dimtheylpentyl, 3,3-dimethylhexyl, 4,4-dimethylhexyl, 2-ethylpentyl, 3-ethylpentyl, 2-ethylhexyl, 3-ethylhexyl, 4-ethylhexyl, 2-methyl-2-ethylpentyl, 2-methyl-3-ethylpentyl, 2-methyl-4-ethylpentyl, 2-methyl-2-ethylhexyl, 2-methyl-3-ethylhexyl, 2-methyl-4-ethylhexyl, 2,2-diethylpentyl, 3,3-diethylhexyl, 2,2-diethylhexyl, 3,3-diethylhexyl and the like. Alkyl groups included in compounds of this invention may be optionally substituted with one or more substituents. Examples of substituents include, but are not limited to, amino, alkylamino, alkoxy, alkylsulfanyl, oxo, halo, acyl, nitro, hydroxyl, cyano, aryl, alkylaryl, aryloxy, arylsulfanyl, arylamino, carbocyclyl, carbocyclyloxy, carbocyclylthio, carbocyclylamino, heterocyclyl, heterocyclyloxy, heterocyclylamino, heterocyclylthio, and the like. In addition, any carbon in the alkyl segment may be substituted with oxygen (═O), sulfur (═S), or nitrogen (═NR²², wherein R²² is —H, an alkyl, acetyl, or aralkyl). Lower alkyls are typically preferred for the compounds of this invention.

The term alkylene refers to an alkyl group or a cycloalkyl group that has two points of attachment to two moieties (e.g., {—CH₂—}, —{CH₂CH₂—},

etc., wherein the brackets indicate the points of attachment). Alkylene groups may be substituted or unsubstituted with one or more substituents.

An aralkyl group refers to an aryl group that is attached to another moiety via an alkylene linker. Aralkyl groups can be substituted or unsubstituted with one or more substituents.

The term “alkoxy,” as used herein, refers to an alkyl group which is linked to another moiety though an oxygen atom. Alkoxy groups can be substituted or unsubstituted with one or more substituents.

The term “alkylsulfanyl,” as used herein, refers to an alkyl group which is linked to another moiety though a divalent sulfur atom. Alkylsulfanyl groups can be substituted or unsubstituted with one or more substituents.

The term “arylsulfanyl,” as used herein, refers to an aryl group which is linked to another moiety though a divalent sulfur atom. Arylsulfanyl groups can be substituted or unsubstituted with one or more substituents.

The term “alkyl ester” as used herein, refers to a group represented by the formula —C(O)OR₃₂, wherein R₃₂ is an alkyl group. A lower alkyl ester is a group represented by the formula —C(O)OR₃₂, wherein R₃₂ is a lower alkyl group.

The term “heteroalkyl,” as used herein, refers to an alkyl group which has one or more carbons in the alkyl chain replaced with an —O—, —S— or —NR₂₇—, wherein R₂₇ is H or a lower alkyl. Heteroalkyl groups can be substituted or unsubstituted with one or more substituents.

The term “alkylamino,” as used herein, refers to an amino group in which one hydrogen atom attached to the nitrogen has been replaced by an alkyl group. The term “dialkylamino,” as used herein, refers to an amino group in which two hydrogen atoms attached to the nitrogen have been replaced by alkyl groups, in which the alkyl groups can be the same or different. Alkylamino groups and dialkylamino groups can be substituted or unsubstituted with one or more substituents.

As used herein, the term “alkenyl” means a straight chain or branched, hydrocarbon radical typically having from 2 to 10 carbon atoms and having at least one carbon-carbon double bond. Representative straight chain and branched alkenyls include vinyl, allyl, 1-butenyl, 2-butenyl, isobutylenyl, 1-pentenyl, 2-pentenyl, 3-methyl-1-butenyl, 1-methyl-2-butenyl, 2,3-dimethyl-2-butenyl, 1-hexenyl, 2-hexenyl, 3-hexenyl, 1-heptenyl, 2-heptenyl, 3-heptenyl, 1-octenyl, 2-octenyl, 3-octenyl, 1-nonenyl, 2-nonenyl, 3-nonenyl, 1-decenyl, 2-decenyl, 3-decenyl and the like. Alkenyl groups can be substituted or unsubstituted with one or more substituents.

As used herein, the term “alkynyl” means a straight chain or branched, hydrocarbon radical typically having from 2 to 10 carbon atoms and having at lease one carbon-carbon triple bond. Representative straight chain and branched alkynyls include acetylenyl, propynyl, 1-butynyl, 2-butynyl, 1-pentynyl, 2-pentynyl, 3-methyl-1-butynyl, 4-pentynyl, 1-hexynyl, 2-hexynyl, 5-hexynyl, 1-heptynyl, 2-heptynyl, 6-heptynyl, 1-octynyl, 2-octynyl, 7-octynyl, 1-nonynyl, 2-nonynyl, 8-nonynyl, 1-decynyl, 2-decynyl, 9-decynyl and the like. Alkynyl groups can be substituted or unsubstituted with one or more substituents.

As used herein, the term “cycloalkyl” means a saturated, mono- or polycyclic alkyl radical typically having from 3 to 14 carbon atoms. Representative cycloalkyls include cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl, cycloheptyl, cyclooctyl, cyclononyl, cyclodecyl, adamantly, decahydronaphthyl, octahydropentalene, bicycle[1.1.1]pentanyl, and the like. Cycloalkyl groups can be substituted or unsubstituted with one or more substituents.

As used herein, the term “cycloalkenyl” means a cyclic non-aromatic alkenyl radical having at least one carbon-carbon double bond in the cyclic system and typically having from 5 to 14 carbon atoms. Representative cycloalkenyls include cyclopentenyl, cyclopentadienyl, cyclohexenyl, cyclohexadienyl, cycloheptenyl, cycloheptadienyl, cycloheptatrienyl, cyclooctenyl, cyclooctadienyl, cyclooctatrienyl, cyclooctatetraenyl, cyclononenyl, cyclononadienyl, cyclodecenyl, cyclodecadienyl and the like. Cycloalkenyl groups can be substituted or unsubstituted with one or more substituents.

As used herein, the term “heterocyclyl” means a monocyclic or polycyclic heterocyclic ring (typically having 3- to 14-members) which is either a saturated ring or an unsaturated non-aromatic ring. A 3-membered heterocyclyl can contain up to 3 heteroatoms, and a 4- to 14-membered heterocyclyl can contain from 1 to about 8 heteroatoms. Each heteroatom is independently selected from nitrogen, which can be quaternized; oxygen; and sulfur, including sulfoxide and sulfone. The heterocyclyl may be attached via any heteroatom or carbon atom. Representative heterocyclyls include morpholinyl, thiomorpholinyl, pyrrolidinonyl, pyrrolidinyl, piperidinyl, piperazinyl, hydantoinyl, valerolactamyl, oxiranyl, oxetanyl, tetrahydrofuranyl, tetrahydropyranyl, 4H-pyranyl, tetrahydropyrindinyl, tetrahydropyrimidinyl, tetrahydrothiophenyl, tetrahydrothiopyranyl, and the like. A heteroatom may be substituted with a protecting group known to those of ordinary skill in the art, for example, the hydrogen on a nitrogen may be substituted with a tert-butoxycarbonyl group. Furthermore, the heterocyclyl may be optionally substituted with one or more substituents (including without limitation a halo, an alkyl, a haloalkyl, or aryl). Only stable isomers of such substituted heterocyclic groups are contemplated in this definition.

As used herein, the term “heteroaromatic” or “heteroaryl” means a monocyclic or polycyclic heteroaromatic ring (or radical thereof) comprising carbon atom ring members and one or more heteroatom ring members (such as, for example, oxygen, sulfur or nitrogen). Typically, the heteroaromatic ring has from 5 to about 14 ring members in which at least 1 ring member is a heteroatom selected from oxygen, sulfur and nitrogen. In another embodiment, the heteroaromatic ring is a 5 or 6 membered ring and may contain from 1 to about 4 heteroatoms. In another embodiment, the heteroaromatic ring system has a 7 to 14 ring members and may contain from 1 to about 7 heteroatoms. Representative heteroaryls include pyridyl, furyl, thienyl, pyrrolyl, oxazolyl, imidazolyl, indolizinyl, thiazolyl, isoxazolyl, pyrazolyl, isothiazolyl, pyridazinyl, pyrimidinyl, pyrazinyl, triazinyl, triazolyl, pyridinyl, thiadiazolyl, pyrazinyl, quinolyl, isoquniolyl, indazolyl, benzoxazolyl, benzofuryl, benzothiazolyl, indolizinyl, imidazopyridinyl, isothiazolyl, tetrazolyl, benzimidazolyl, benzoxazolyl, benzothiazolyl, benzothiadiazolyl, benzoxadiazolyl, indolyl, tetrahydroindolyl, azaindolyl, imidazopyridyl, qunizaolinyl, purinyl, pyrrolo[2,3]pyrimidyl, pyrazolo[3,4]pyrimidyl or benzo(b)thienyl and the like. Heteroaryl groups may be optionally substituted with one or more substituents

A heteroaralkyl group refers to a heteroaryl group that is attached to another moiety via an alkylene linker. Heteroaralkyl groups can be substituted or unsubstituted with one or more substituents.

As used herein, the term “N-containing 6-membered aromatic ring” means an optionally substituted 6-membered aromatic ring that contains one or more N atoms. In one embodiment, the ring contains one N atom. In another embodiment, the ring contains two N atoms.

As used herein, the term “halogen” or “halo” means —F, —Cl, —Br or —I.

As used herein, the term “haloalkyl” means an alkyl group in which one or more —H is replaced with a halo group. Examples of haloalkyl groups include —CF₃, —CHF₂, —CCl₃, —CH₂CH₂Br, —CH₂CH(CH₂CH₂Br)CH₃, —CHICH₃, and the like.

As used herein, the term “haloalkoxy” means an alkoxy group in which one or more —H is replaced with a halo group. Examples of haloalkoxy groups include —OCF₃ and —OCHF₂.

The terms “bioisostere” and “bioisosteric replacement” have the same meanings as those generally recognized in the art. Bioisosteres are atoms, ions, or molecules in which the peripheral layers of electrons can be considered substantially identical. The term bioisostere is usually used to mean a portion of an overall molecule, as opposed to the entire molecule itself. Bioisosteric replacement involves using one bioisostere to replace another with the expectation of maintaining or slightly modifying the biological activity of the first bioisostere. The bioisosteres in this case are thus atoms or groups of atoms having similar size, shape and electron density. Preferred bioisosteres of esters, amides or carboxylic acids are compounds containing two sites for hydrogen bond acceptance. In one embodiment, the ester, amide or carboxylic acid bioisostere is a 5-membered monocyclic heteroaryl ring, such as an optionally substituted 1H-imidazolyl, an optionally substituted oxazolyl, 1H-tetrazolyl, [1,2,4]triazolyl, or an optionally substituted [1,2,4]oxadiazolyl.

As used herein, the terms “subject”, “patient” and “animal”, are used interchangeably and include, but are not limited to, a cow, monkey, horse, sheep, pig, mini pig, chicken, turkey, quail, cat, dog, mouse, rat, rabbit, guinea pig and human. The preferred subject, patient or animal is a human.

As used herein, the term “lower” refers to a group having up to four carbon atoms. For example, a “lower alkyl” refers to an alkyl radical having from 1 to 4 carbon atoms, and a “lower alkenyl” or “lower alkynyl” refers to an alkenyl or alkynyl radical having from 2 to 4 carbon atoms, respectively. A lower alkoxy or a lower alkylsulfanyl refers to an alkoxy or an alkylsulfanyl having from 1 to 4 carbon atoms. Lower substituents are typically preferred.

Where a particular substituent, such as an alkyl substituent, occurs multiple times in a given structure or moiety, the identity of the substituent is independent in each case and may be the same as or different from other occurrences of that substituent in the structure or moiety. Furthermore, individual substituents in the specific embodiments and exemplary compounds of this invention are preferred in combination with other such substituents in the compounds of this invention, even if such individual substituents are not expressly noted as being preferred or not expressly shown in combination with other substituents.

The compounds of the invention are defined herein by their chemical structures and/or chemical names. Where a compound is referred to by both a chemical structure and a chemical name, and the chemical structure and chemical name conflict, the chemical structure is determinative of the compound's identity.

Suitable substituents for an alkyl, alkoxy, alkylsulfanyl, alkylamino, dialkylamino, alkylene, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclyl, aryl, aralkyl, heteroaryl, and heteroaralkyl groups include any substituent which will form a stable compound of the invention. Examples of substituents for an alkyl, alkoxy, alkylsulfanyl, alkylamino, dialkylamino, alkylene, alkenyl, alkynyl, cycloalkyl, cycloalkenyl, heterocyclyl, aryl, aralkyl, heteroaryl, and heteroaralkyl include an alkyl, an alkoxy, an alkylsulfanyl, an alkylamino, a dialkylamino, an alkenyl, an alkynyl, a cycloalkyl, a cycloalkenyl, a heterocyclyl, an aryl, a heteroaryl, an aralkyl, a heteraralkyl, a haloalkyl, —C(O)NR₂₃R₂₄, —NR₂₅C(O)R₂₆, halo, —OR₂₅, cyano, nitro, haloalkoxy, —C(O)R₂₅, —NR₂₃R₂₄, —SR₂₅, —C(O)OR₂₅, —OC(O)R₂₅, —NR₂₅C(O)NR₂₃R₂₄, —OC (O)NR₂₃R₂₄, —NR₂₅C(O)OR₂₆, —S(O)_(p)R₂₅, or —S(O)_(p)NR₂₃R₂₄, wherein R₂₃ and R₂₄, for each occurrence are, independently, H, an alkyl, an alkenyl, an alkynyl, a cycloalkyl, a cycloalkenyl, a heterocyclyl, an aryl, a heteroaryl, an aralkyl, or a heteraralkyl; or R₂₃ and R₂₄ taken together with the nitrogen to which they are attached is a heterocyclyl or a heteroaryl; and R₂₅ and R₂₆ for each occurrence are, independently, H, an alkyl, an alkenyl, an alkynyl, a cycloalkyl, a cycloalkenyl, a heterocyclyl, an aryl, a heteroaryl, an aralkyl, or a heteraralkyl;

In addition, alkyl, cycloalkyl, alkylene, a heterocyclyl, and any saturated portion of a alkenyl, cycloalkenyl, alkynyl, aralkyl, and heteroaralkyl groups, may also be substituted with ═O, ═S, ═N—R₂₂.

When a heterocyclyl, heteroaryl, or heteroaralkyl group contains a nitrogen atom, it may be substituted or unsubstituted. When a nitrogen atom in the aromatic ring of a heteroaryl group has a substituent the nitrogen may be a quaternary nitrogen.

Choices and combinations of substituents and variables envisioned by this invention are only those that result in the formation of stable compounds. The term “stable”, as used herein, refers to compounds which possess stability sufficient to allow manufacture and which maintains the integrity of the compound for a sufficient period of time to be useful for the purposes detailed herein (e.g., therapeutic or prophylactic administration to a subject). Typically, such compounds are stable at a temperature of 40° C. or less, in the absence of excessive moisture, for at least one week. Such choices and combinations will be apparent to those of ordinary skill in the art and may be determined without undue experimentation.

Unless indicated otherwise, the compounds of the invention containing reactive functional groups (such as, without limitation, carboxy, hydroxy, and amino moieties) also include protected derivatives thereof. “Protected derivatives” are those compounds in which a reactive site or sites are blocked with one ore more protecting groups. Suitable protecting groups for carboxy moieties include benzyl, tert-butyl, and the like. Suitable protecting groups for amino and amido groups include acetyl, tert-butoxycarbonyl, benzyloxycarbonyl, and the like. Suitable protecting groups for hydroxy include benzyl, trimethyl silyl (TMS) and the like. Other suitable protecting groups are well known to those of ordinary skill in the art and include those found in T. W. Greene, Protecting Groups in Organic Synthesis, John Wiley & Sons, Inc. 1981, the entire teachings of which are incorporated herein by reference.

As used herein, the term “compound(s) of this invention” and similar terms refers to a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof and also include protected derivatives thereof.

As used herein and unless otherwise indicated, the term “prodrug” means a derivative of a compound that can hydrolyze, oxidize, or otherwise react under biological conditions (in vitro or in vivo) to provide a compound of this invention. Prodrugs may only become active upon such reaction under biological conditions, but they may have activity in their unreacted forms. Examples of prodrugs contemplated in this invention include, but are not limited to, analogs or derivatives of compounds of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1 that comprise biohydrolyzable moieties such as biohydrolyzable amides, biohydrolyzable esters, biohydrolyzable carbamates, biohydrolyzable carbonates, biohydrolyzable ureides, and biohydrolyzable phosphate analogues. Other examples of prodrugs include derivatives of compounds of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or of Table 1 that comprise —NO, —NO₂, —ONO, or —ONO₂ moieties. Prodrugs can typically be prepared using well-known methods, such as those described by 1 BURGER'S MEDICINAL CHEMISTRY AND DRUG DISCOVERY (1995) 172-178, 949-982 (Manfred E. Wolff ed., 5^(th) ed), the entire teachings of which are incorporated herein by reference.

As used herein and unless otherwise indicated, the terms “biohydrolyzable amide”, “biohydrolyzable ester”, “biohydrolyzable carbamate”, “biohydrolyzable carbonate”, “biohydrolyzable ureide” and “biohydrolyzable phosphate analogue” mean an amide, ester, carbamate, carbonate, ureide, or phosphate analogue, respectively, that either: 1) does not destroy the biological activity of the compound and confers upon that compound advantageous properties in vivo, such as uptake, duration of action, or onset of action; or 2) is itself biologically inactive but is converted in vivo to a biologically active compound. Examples of biohydrolyzable amides include, but are not limited to, lower alkyl amides, α-amino acid amides, alkoxyacyl amides, and alkylaminoalkylcarbonyl amides. Examples of biohydrolyzable esters include, but are not limited to, lower alkyl esters, alkoxyacyloxy esters, alkyl acylamino alkyl esters, and choline esters. Examples of biohydrolyzable carbamates include, but are not limited to, lower alkylamines, substituted ethylenediamines, aminoacids, hydroxyalkylamines, heterocyclic and heteroaromatic amines, and polyether amines.

As used herein, the term “pharmaceutically acceptable salt,” is a salt formed from an acid and a basic group of one of the compounds of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or of Table 1. Illustrative salts include, but are not limited, to sulfate, citrate, acetate, oxalate, chloride, bromide, iodide, nitrate, bisulfate, phosphate, acid phosphate, isonicotinate, lactate, salicylate, acid citrate, tartrate, oleate, tannate, pantothenate, bitartrate, ascorbate, succinate, maleate, gentisinate, fumarate, gluconate, glucaronate, saccharate, formate, benzoate, glutamate, methanesulfonate, ethanesulfonate, benzenesulfonate, p-toluenesulfonate, and pamoate (i.e., 1,1′-methylene-bis-(2-hydroxy-3-naphthoate)) salts. The term “pharmaceutically acceptable salt” also refers to a salt prepared from a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1 having an acidic functional group, such as a carboxylic acid functional group, and a pharmaceutically acceptable inorganic or organic base. Suitable bases include, but are not limited to, hydroxides of alkali metals such as sodium, potassium, and lithium; hydroxides of alkaline earth metal such as calcium and magnesium; hydroxides of other metals, such as aluminum and zinc; ammonia, and organic amines, such as unsubstituted or hydroxy-substituted mono-, di-, or trialkylamines; dicyclohexylamine; tributyl amine; pyridine; N-methyl, N-ethylamine; diethylamine; triethylamine; mono-, bis-, or tris-(2-hydroxy-lower alkyl amines), such as mono-, bis-, or tris-(2-hydroxyethyl)-amine, 2-hydroxy-tert-butylamine, or tris-(hydroxymethyl)methylamine, N,N,-di-lower alkyl-N-(hydroxy lower alkyl)-amines, such as N,N-dimethyl-N-(2-hydroxyethyl)-amine, or tri-(2-hydroxyethyl)amine; N-methyl-D-glucamine; and amino acids such as arginine, lysine, and the like. The term “pharmaceutically acceptable salt” also refers to a salt prepared from a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1 having a basic functional group, such as an amino functional group, and a pharmaceutically acceptable inorganic or organic acid. Suitable acids include, but are not limited to, hydrogen sulfate, citric acid, acetic acid, oxalic acid, hydrochloric acid, hydrogen bromide, hydrogen iodide, nitric acid, phosphoric acid, isonicotinic acid, lactic acid, salicylic acid, tartaric acid, ascorbic acid, succinic acid, maleic acid, besylic acid, fumaric acid, gluconic acid, glucaronic acid, saccharic acid, formic acid, benzoic acid, glutamic acid, methanesulfonic acid, ethanesulfonic acid, benzenesulfonic acid, and p-toluenesulfonic acid.

When a disclosed compound is named or depicted by structure, it is to be understood that solvates (e.g., hydrates) of the compound or its pharmaceutically acceptable salts are also included. “Solvates” refer to crystalline forms wherein solvent molecules are incorporated into the crystal lattice during crystallization. Solvate may include water or nonaqueous solvents such as ethanol, isopropanol, DMSO, acetic acid, ethanolamine, and EtOAc. Solvates, wherein water is the solvent molecule incorporated into the crystal lattice, are typically referred to as “hydrates”. Hydrates include a stoichiometric or non-stoichiometric amount of water bound by non-covalent intermolecular forces.

When a disclosed compound is named or depicted by structure, it is to be understood that the compound, including solvates thereof, may exist in crystalline forms, non-crystalline forms or a mixture thereof. The compounds or solvates may also exhibit polymorphism (i.e. the capacity to occur in different crystalline forms). These different crystalline forms are typically known as “polymorphs.” It is to be understood that when named or depicted by structure, the disclosed compounds and solvates (e.g., hydrates) also include all polymorphs thereof. As used herein, the term “polymorph” means solid crystalline forms of a compound of the present invention or complex thereof. Different polymorphs of the same compound can exhibit different physical, chemical and/or spectroscopic properties. Different physical properties include, but are not limited to stability (e.g., to heat or light), compressibility and density (important in formulation and product manufacturing), and dissolution rates (which can affect bioavailability). Differences in stability can result from changes in chemical reactivity (e.g., differential oxidation, such that a dosage form discolors more rapidly when comprised of one polymorph than when comprised of another polymorph) or mechanical characteristics (e.g., tablets crumble on storage as a kinetically favored polymorph converts to thermodynamically more stable polymorph) or both (e.g., tablets of one polymorph are more susceptible to breakdown at high humidity). Different physical properties of polymorphs can affect their processing. For example, one polymorph might be more likely to form solvates or might be more difficult to filter or wash free of impurities than another due to, for example, the shape or size distribution of particles of it. In addition, one polymorph may spontaneously convert to another polymorph under certain conditions.

When a disclosed compound is named or depicted by structure, it is to be understood that clathrates (“inclusion compounds”) of the compound or its pharmaceutically acceptable salts, solvates or polymorphs are also included. As used herein, he term “clathrate” means a compound of the present invention or a salt thereof in the form of a crystal lattice that contains spaces (e.g., channels) that have a guest molecule (e.g., a solvent or water) trapped within.

As used herein, the term “asthma” means a pulmonary disease, disorder or condition characterized by reversible airway obstruction, airway inflammation, and increased airway responsiveness to a variety of stimuli.

“Immunosuppression” refers to impairment of any component of the immune system resulting in decreased immune function. This impairment may be measured by any conventional means including whole blood assays of lymphocyte function, detection of lymphocyte proliferation and assessment of the expression of T cell surface antigens. The antisheep red blood cell (SRBC) primary (IgM) antibody response assay (usually referred to as the plaque assay) is one specific method. This and other methods are described in Luster, M. I., Portier, C., Pait, D. G., White, K. L., Jr., Gennings, C., Munson, A. E., and Rosenthal, G. J. (1992). “Risk Assessment in Immunotoxicology I: Sensitivity and Predictability of Immune Tests.” Fundam. Appl. Toxicol., 18, 200-210. Measuring the immune response to a T-cell dependent immunogen is another particularly useful assay (Dean, J. H., House, R. V., and Luster, M. I. (2001). “Immunotoxicology: Effects of, and Responses to, Drugs and Chemicals.” In Principles and Methods of Toxicology: Fourth Edition (A. W. Hayes, Ed.), pp. 1415-1450, Taylor & Francis, Philadelphia, Pa.).

The compounds of this invention can be used to treat subjects with immune disorders. As used herein, the term “immune disorder” and like terms means a disease, disorder or condition caused by the immune system of an animal, including autoimmune disorders. Immune disorders include those diseases, disorders or conditions that have an immune component and those that are substantially or entirely immune system-mediated. Autoimmune disorders are those wherein the animal's own immune system mistakenly attacks itself, thereby targeting the cells, tissues, and/or organs of the animal's own body. For example, the autoimmune reaction is directed against the nervous system in multiple sclerosis and the gut in Crohn's disease. In other autoimmune disorders such as systemic lupus erythematosus (lupus), affected tissues and organs may vary among individuals with the same disease. One person with lupus may have affected skin and joints whereas another may have affected skin, kidney, and lungs. Ultimately, damage to certain tissues by the immune system may be permanent, as with destruction of insulin-producing cells of the pancreas in Type 1 diabetes mellitus. Specific autoimmune disorders that may be ameliorated using the compounds and methods of this invention include without limitation, autoimmune disorders of the nervous system (e.g., multiple sclerosis, myasthenia gravis, autoimmune neuropathies such as Guillain-Barré, and autoimmune uveitis), autoimmune disorders of the blood (e.g., autoimmune hemolytic anemia, pernicious anemia, and autoimmune thrombocytopenia), autoimmune disorders of the blood vessels (e.g., temporal arteritis, anti-phospholipid syndrome, vasculitides such as Wegener's granulomatosis, and Behcet's disease), autoimmune disorders of the skin (e.g., psoriasis, dermatitis herpetiformis, pemphigus vulgaris, and vitiligo), autoimmune disorders of the gastrointestinal system (e.g., Crohn's disease, ulcerative colitis, primary biliary cirrhosis, and autoimmune hepatitis), autoimmune disorders of the endocrine glands (e.g., Type 1 or immune-mediated diabetes mellitus, Grave's disease. Hashimoto's thyroiditis, autoimmune oophoritis and orchitis, and autoimmune disorder of the adrenal gland); and autoimmune disorders of multiple organs (including connective tissue and musculoskeletal system diseases) (e.g., rheumatoid arthritis, systemic lupus erythematosus, scleroderma, polymyositis, dermatomyositis, spondyloarthropathies such as ankylosing spondylitis, and Sjogren's syndrome). In addition, other immune system mediated diseases, such as graft-versus-host disease and allergic disorders, are also included in the definition of immune disorders herein. Because a number of immune disorders are caused by inflammation, there is some overlap between disorders that are considered immune disorders and inflammatory disorders. For the purpose of this invention, in the case of such an overlapping disorder, it may be considered either an immune disorder or an inflammatory disorder. “Treatment of an immune disorder” herein refers to administering a compound or a composition of the invention to a subject, who has an immune disorder, a symptom of such a disease or a predisposition towards such a disease, with the purpose to cure, relieve, alter, affect, or prevent the autoimmune disorder, the symptom of it, or the predisposition towards it.

As used herein, the term “allergic disorder” means a disease, condition or disorder associated with an allergic response against normally innocuous substances. These substances may be found in the environment (such as indoor air pollutants and aeroallergens) or they may be non-environmental (such as those causing dermatological or food allergies). Allergens can enter the body through a number of routes, including by inhalation, ingestion, contact with the skin or injection (including by insect sting). Many allergic disorders are linked to atopy, a predisposition to generate the allergic antibody IgE. Because IgE is able to sensitize mast cells anywhere in the body, atopic individuals often express disease in more than one organ. For the purpose of this invention, allergic disorders include any hypersensitivity that occurs upon re-exposure to the sensitizing allergen, which in turn causes the release of inflammatory mediators. Allergic disorders include without limitation, allergic rhinitis (e.g., hay fever), sinusitis, rhinosinusitis, chronic or recurrent otitis media, drug reactions, insect sting reactions, latex reactions, conjunctivitis, urticaria, anaphylaxis and anaphylactoid reactions, atopic dermatitis, asthma and food allergies.

The compounds of this invention can be used to prevent or to treat subjects with inflammatory disorders. As used herein, an “inflammatory disorder” means a disease, disorder or condition characterized by inflammation of body tissue or having an inflammatory component. These include local inflammatory responses and systemic inflammation. Examples of such inflammatory disorders include: transplant rejection, including skin graft rejection; chronic inflammatory disorders of the joints, including arthritis, rheumatoid arthritis, osteoarthritis and bone diseases associated with increased bone resorption; inflammatory bowel diseases such as ileitis, ulcerative colitis, Barrett's syndrome, and Crohn's disease; inflammatory lung disorders such as asthma, adult respiratory distress syndrome, and chronic obstructive airway disease; inflammatory disorders of the eye including corneal dystrophy, trachoma, onchocerciasis, uveitis, sympathetic ophthalmitis and endophthalmitis; chronic inflammatory disorders of the gums, including gingivitis and periodontitis; tuberculosis; leprosy; inflammatory diseases of the kidney including uremic complications, glomerulonephritis and nephrosis; inflammatory disorders of the skin including sclerodermatitis, psoriasis and eczema; inflammatory diseases of the central nervous system, including chronic demyelinating diseases of the nervous system, multiple sclerosis, AIDS-related neurodegeneration and Alzheimer's disease, infectious meningitis, encephalomyelitis, Parkinson's disease, Huntington's disease, amyotrophic lateral sclerosis and viral or autoimmune encephalitis; autoimmune disorders, immune-complex vasculitis, systemic lupus and erythematodes; systemic lupus erythematosus (SLE); and inflammatory diseases of the heart such as cardiomyopathy, ischemic heart disease hypercholesterolemia, atherosclerosis; as well as various other diseases with significant inflammatory components, including preeclampsia; chronic liver failure, brain and spinal cord trauma, and cancer. There may also be a systemic inflammation of the body, exemplified by gram-positive or gram negative shock, hemorrhagic or anaphylactic shock, or shock induced by cancer chemotherapy in response to pro-inflammatory cytokines, e.g., shock associated with pro-inflammatory cytokines. Such shock can be induced, e.g., by a chemotherapeutic agent used in cancer chemotherapy. “Treatment of an inflammatory disorder” herein refers to administering a compound or a composition of the invention to a subject, who has an inflammatory disorder, a symptom of such a disorder or a predisposition towards such a disorder, with the purpose to cure, relieve, alter, affect, or prevent the inflammatory disorder, the symptom of it, or the predisposition towards it.

An “effective amount” is the quantity of compound in which a beneficial outcome is achieved when the compound is administered to a subject or alternatively, the quantity of compound that possess a desired activity in-vivo or in-vitro. In the case of inflammatory disorders and autoimmune disorders, a beneficial clinical outcome includes reduction in the extent or severity of the symptoms associated with the disease or disorder and/or an increase in the longevity and/or quality of life of the subject compared with the absence of the treatment. The precise amount of compound administered to a subject will depend on the type and severity of the disease or condition and on the characteristics of the subject, such as general health, age, sex, body weight and tolerance to drugs. It will also depend on the degree, severity and type of inflammatory disorder, autoimmune disorder, allergic disorder, or the degree of immunosuppression sought. The skilled artisan will be able to determine appropriate dosages depending on these and other factors. Effective amounts of the disclosed compounds typically range between about 1 mg/m² per day and about 10 grams/m² per day, and preferably between 10 mg/m² per day and about 1 gram/m².

The compounds of the invention may contain one or more chiral centers and/or double bonds and, therefore, may exist as stereoisomers, such as double-bond isomers (i.e., geometric isomers), enantiomers, or diastereomers. According to this invention, the chemical structures depicted herein, including the compounds of this invention, encompass all of the corresponding compounds' enantiomers and stereoisomers, that is, both the stereomerically pure form (e.g., geometrically pure, enantiomerically pure, or diastereomerically pure) and enantiomeric, diastereomeric, and geometric isomeric mixtures. In some cases, one enantiomer, diastereomer, or geometric isomer will possess superior activity or an improved toxicity or kinetic profile compared to others. In those cases, such enantiomers, diastereomers, and geometric isomers of a compound of this invention are preferred.

The term “inhibit production of IL-2” and like terms means inhibiting IL-2 synthesis (e.g. by inhibiting transcription (mRNA expression), or translation (protein expression)) and/or inhibiting IL-2 secretion in a cell that has the ability to produce and/or secrete IL-2 (e.g., T lymphocyte). Likewise, the term “inhibiting production of IL-4, IL-5, IL-13, GM-CSF, TNF-α or INF-γ means inhibiting the synthesis (e.g. by inhibiting transcription, or translation) and/or inhibiting the secretion in a cell that has the ability to produce and/or secrete these cytokines.

As used herein, a composition that “substantially” comprises a compound means that the composition contains more than about 80% by weight, more preferably more than about 90% by weight, even more preferably more than about 95% by weight, and most preferably more than about 97% by weight of the compound.

As used herein, a composition that is “substantially free” of a compound means that the composition contains less than about 20% by weight, more preferably less than about 10% by weight, even more preferably less than about 5% by weight, and most preferably less than about 3% by weight of the compound.

As used herein, a reaction that is “substantially complete” means that the reaction contains more than about 80% by weight of the desired product, more preferably more than about 90% by weight of the desired product, even more preferably more than about 95% by weight of the desired product, and most preferably more than about 97% by weight of the desired product.

As used herein, a racemic mixture means about 50% of one enantiomer and about 50% of is corresponding enantiomer relative to all chiral centers in the molecule. The invention encompasses all enantiomerically-pure, enantiomerically-enriched, diastereomerically pure, diastereomerically enriched, and racemic mixtures of the compounds of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1.

Enantiomeric and diastereomeric mixtures can be resolved into their component enantiomers or stereoisomers by well known methods, such as chiral-phase gas chromatography, chiral-phase high performance liquid chromatography, crystallizing the compound as a chiral salt complex, or crystallizing the compound in a chiral solvent. Enantiomers and diastereomers can also be obtained from diastereomerically- or enantiomerically-pure intermediates, reagents, and catalysts by well known asymmetric synthetic methods.

When administered to a patient, e.g., to a non-human animal for veterinary use or for improvement of livestock, or to a human for clinical use, the compounds of the invention are typically administered in isolated form or as the isolated form in a pharmaceutical composition. As used herein, “isolated” means that the compounds of the invention are separated from other components of either (a) a natural source, such as a plant or cell, preferably bacterial culture, or (b) a synthetic organic chemical reaction mixture. Preferably, via conventional techniques, the compounds of the invention are purified. As used herein, “purified” means that when isolated, the isolate contains at least 95%, preferably at least 98%, of a single compound of the invention by weight of the isolate.

Only those choices and combinations of substituents that result in a stable structure are contemplated. Such choices and combinations will be apparent to those of ordinary skill in the art and may be determined without undue experimentation.

The invention can be understood more fully by reference to the following detailed description and illustrative examples, which are intended to exemplify non-limiting embodiments of the invention.

SPECIFIC EMBODIMENTS

The invention relates to compounds and pharmaceutical compositions that are particularly useful for immunosuppression or to treat or prevent inflammatory conditions, immune disorders, and allergic disorders.

In one embodiment, the invention relates to compounds of structural formula (I):

-   -   or a pharmaceutically acceptable salt, solvate, clathrate, or         prodrug thereof, wherein:     -   X₁ and X₂ are, independently, CH or N;     -   one of X₃ or X₆ is NR₂₉, and the other is CH₂, CHR₂, or C(R₂)₂;     -   L is a linker selected from the group consisting of —NHCH₂—,         —CH₂NH—, —NR₅—C(O)—, —C(O)—NR₅—, —NR₅—C(S)—, —C(S)—NR₅—,         —NR₅—C(NR₈)—, —C(NR₈)—NR₅—, —NR₅C(S)NR₅—, —NR₅C(NR₈)NR₅—, or         —NR₅CR^(a)R^(b)NR₅—;     -   Y is a substituted phenyl, provided that Y is not substituted         with —NHC(O)R₃₀;     -   R₁ is H or a substituent;     -   R₂, for each occurrence is, independently, a substituent,         provided that R₂ is not ═O or ═C(R₂₈)₂;     -   R₄ is a substituent;     -   R^(a) and R^(b), for each occurrence, are independently, H, an         optionally substituted alkyl, an optionally substituted alkenyl,         an optionally substituted alkynyl, an optionally substituted         cycloalkyl, an optionally substituted cycloalkenyl, an         optionally substituted heterocyclyl, an optionally substituted         aryl, an optionally substituted heteroaryl, an optionally         substituted aralkyl, an optionally substituted heteraralkyl,         cyano, nitro, halo, —OR₅, —SR₅, —NR₆R₇, —C(O)NR₆R₇, —NR₅C(O)R₅,         —C(O)R₅, —C(O)OR₅, —OC(O)R₅, —C(O)SR₅, —SC(O)R₅, —C(S)NR₆R₇,         —NR₅C(S)R₅, —C(S)R₅, —C(S)OR₅, —OC(S)R₅, —C(S)SR₅, —SC(S)R₅,         —C(NR₈)NR₆R₇, —NR₅C(NR₈)R₅, —C(NR₈)R₅, —C(NR₈)OR₅, —OC(NR₈)R₅,         —C(NR₈)SR₅, —SC(NR₈)R₅, —OC(O)OR₅, —OC(O)NR₆R₇, —NR₅C(O)OR₅,         —NR₅C(O)NR₆R₇, —SC(O)OR₅, —SC(O)NR₆R₇, —SC(O)SR₅, —NR₅C(O)SR₅,         —OC(O)SR₅, —OC(S)OR₅, —OC(S)NR₆R₇, —NR₅C(S)OR₅, —NR₅C(S)NR₆R₇,         —SC(S)OR₅, —SC(S)NR₆R₇, —SC(S)SR₅, —NR₅C(S)SR₅, —OC(S)SR₅,         —OC(NR₈)OR₅, —OC(NR₈)NR₆R₇, —NR₅C(NR₈)OR₅, —NR₅C(NR₈)NR₆R₇,         —SC(NR₈)OR₅, —SC(NR₈)NR₆R₇, —SC(NR₈)SR₅, —NR₅C(NR₈)SR₅, or         —OC(NR₈)SR₅;     -   R₅, for each occurrence, is independently, H, an optionally         substituted alkyl, an optionally substituted alkenyl, an         optionally substituted alkynyl, an optionally substituted         cycloalkyl, an optionally substituted cycloalkenyl, an         optionally substituted heterocyclyl, an optionally substituted         aryl, an optionally substituted heteroaryl, an optionally         substituted aralkyl, or an optionally substituted heteraralkyl;     -   R₆ and R₇, for each occurrence are, independently, H, an         optionally substituted alkyl, an optionally substituted alkenyl,         an optionally substituted alkynyl, an optionally substituted         cycloalkyl, an optionally substituted cycloalkenyl, an         optionally substituted heterocyclyl, an optionally substituted         aryl, an optionally substituted heteroaryl, an optionally         substituted aralkyl, or an optionally substituted heteraralkyl;         or R₆ and R₇ taken together with the nitrogen to which they are         attached are an optionally substituted heterocyclyl or         optionally substituted heteroaryl;     -   R₈, for each occurrence, is independently —H, a halo, an alkyl,         —OR₅, —NR₆R₇, —C(O)R₅, —C(O)OR₅, or —C(O)NR₆R₇;     -   R₂₈, for each occurrence is, independently, H or a substituent;     -   R₂₉ is —C(O)R₃₀ or a N-containing 6-membered aromatic ring;     -   R₃₀ is —H, —OR₅, —SR₅, —NR₆R₇, an optionally substituted alkyl,         an optionally substituted alkenyl, an optionally substituted         alkynyl, an optionally substituted cycloalkyl, an optionally         substituted cycloalkenyl, an optionally substituted         heterocyclyl, an optionally substituted aryl, an optionally         substituted heteroaryl, an optionally substituted aralkyl, or an         optionally substituted heteraralkyl;     -   m is 0, 1, or 2; and     -   n is 0, 1 or 2.

In one aspect, the compound of formula (I) is not:

-   5-[4-(2,6-difluoro-benzoylamino)-phenyl]-4-methyl-2,4-dihydro-2H-pyridine-1-carboxylic     acid ethyl ester; -   Ethyl     3-(4-(2,6-difluorobenzamido)phenyl)-4-methyl-5,6-dihydropyridine-1(2H)-carboxylate; -   Ethyl     4-(4-(2,6-difluorobenzamido)phenyl)-3-methyl-5,6-dihydropyridine-1(2H)-carboxylate; -   2,6-Difluoro-N-[4-(1-methoxycarbonyl-4-thiazol-2-yl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-[4-(1-methoxycarbonyl-4-isopropyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-[4-(1-methoxycarbonyl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-[4-(1-allyloxycarbonyl-3-methyl-1,2,5,6-tetrahydro-pyridin-4-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-[4-(1-phenyloxycarbonyl-3-methyl-1,2,5,6-tetrahydro-pyridin-4-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-[4-(1-acetyl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-[4-(1-cyclopropylcarbonyl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; -   2,6-Difluoro-N-{4-[1-(pyridine-3-carbonyl)-cyclopropanecarbonyl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl]-phenyl}-benzamide;     or -   2,6-Difluoro-N-[4-(1-pyrimidin-2-yl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide.

In another embodiment, the invention relates to compounds represented by formula (IIA) or (IIB):

-   -   or a pharmaceutically acceptable salt, solvate, clathrate, or         prodrug thereof, wherein     -   R₃₁ is halo, an optionally substituted alkyl, an optionally         substituted alkenyl, an optionally substituted alkynyl, an         optionally substituted cycloalkyl, an optionally substituted         cycloalkenyl, an optionally substituted heterocyclyl, an         optionally substituted aryl, an optionally substituted         heteroaryl, an optionally substituted aralkyl, or an optionally         substituted heteraralkyl;     -   p is 1, 2, 3, 4 or 5; and     -   R₁, R₃₀, L, X₁, and X₂ are defined as for formula (I).

In another embodiment, the invention relates to compounds represented by formula (IIIA) or (IIIB):

or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof, wherein R₁, R₃₀, L, X₁, and X₂ are defined as for formula (I) and R₃₁ is defined as for formulas (IIA) and (IIB).

In another embodiment, the invention relates to compounds represented by formula (IVA) or (IVB):

-   -   or a pharmaceutically acceptable salt, solvate, clathrate, or         prodrug thereof, wherein     -   each of X₇, X₈, X₉, X₁₀, and X₁₁ are independently CH, CR₂, or         N, provided that at least one of X₇, X₈, X₉, X₁₀, or X₁₁ is N;     -   R₃₁ is halo, an optionally substituted alkyl, an optionally         substituted alkenyl, an optionally substituted alkynyl, an         optionally substituted cycloalkyl, an optionally substituted         cycloalkenyl, an optionally substituted heterocyclyl, an         optionally substituted aryl, an optionally substituted         heteroaryl, an optionally substituted aralkyl, or an optionally         substituted heteraralkyl;     -   p is 1, 2, 3, 4 or 5; and     -   R₁, L, X₁, and X₂ are defined as for formula (I)

In another embodiment, the invention relates to compounds represented by formula (VA) or (VB):

or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof, wherein R₁, L, X₁, and X₂ are defined as for formula (I) and X₇, X₈, X₉, X₁₀, and X₁₁ are defined as for formulas (IVA) and (IVB).

In some embodiments of the compounds represented by formula (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), R₁ is selected from the group consisting of a halo, an optionally substituted alkyl, an optionally substituted alkenyl, an optionally substituted alkynyl, an optionally substituted cycloalkyl, an optionally substituted cycloalkenyl, an optionally substituted heterocyclyl, an optionally substituted aryl, an optionally substituted heteroaryl, an optionally substituted aralkyl, or an optionally substituted heteraralkyl. In one aspect, R₁ is selected from the group consisting of halo or an optionally substituted lower alkyl. In one aspect, R₁ is selected from the group consisting of —CH₂OAc, CH₃, or Cl.

In some embodiments of the compounds represented by formula (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), L is —NHCH₂—, —CH₂NH—, —NH—C(O)—, or —C(O)—NH—. In one aspect, L is —NHC(O)—.

In some embodiments of the compounds represented by formula (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), X₁ and X₂ are both CH.

In some embodiments of the compounds represented by formula (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), X₁ and X₂ are both N.

In some embodiments of the compounds represented by formula (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), X₁ is N and X₂ is CH.

In some embodiments of the compounds represented by formula (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), X₂ is N and X₁ is CH.

In some embodiments of the compounds represented by formula (I), Y is substituted with one to two substituents. In one aspect, the one to two substituents are each independently a lower alkyl or a halo. In another aspect, Y is a difluorophenyl. In a further aspect, Y is 2,6-difluorophenyl.

In some embodiments of the compounds represented by formula (I), X₃ is CH₂ and X₆ is NR₂₉.

In some embodiments of the compounds represented by formula (I), X₆ is CH₂ and X₃ is NR₂₉.

In some embodiments of the compounds represented by formula (I), m is 0.

In some embodiments of the compounds represented by formula (I), Y is a difluorophenyl;

L is —NHC(O)—; R₁ is selected from the group consisting of halo or an optionally substituted lower alkyl; and m is 0.

In some embodiments of the compounds represented by formula (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), each R₃₁ is independently a lower alkyl or a halo. In one aspect, R₃₁ is halo. In one aspect, R₃₁ is F.

In some embodiments of the compounds represented by formula (IIA), (IIB), (IVA), or (IVB), p is 1 or 2. In one aspect, each R₃₁ is independently a lower alkyl or a halo. In one aspect, R₃₁ is halo. In one aspect, R₃₁ is F.

In some embodiments of the compounds represented by formula (IIIA), (IIIB), (VA), or (VB), each R₃₁ is independently a lower alkyl or a halo. In one aspect, each R₃₁ is halo. In another aspect, each R₃₁ is F.

In some embodiments of the compounds represented by formula (IIA), (IIB), (IIIA), or (IIIB), R₃₀ is selected from the group consisting of —OR₅, optionally substituted alkyl, optionally substituted aryl, and optionally substituted heteroaryl. In one aspect, R₃₀ is selected from the group consisting of —OR₅ and optionally substituted heteroaryl. In another aspect, R₃₀ is —OR₅ and R₅ is a lower alkyl. In a further aspect, R₃₀ is a pyridine ring.

In some embodiments of the compounds represented by formula (IVA), (IVB), (VA), or (VB), two of X₇, X₈, X₉, X₁₀, and X₁₁ are N. In one aspect, X₇ and X₁₁ are both N.

In some embodiments of the compounds represented by formula (IVA), (IVB), (VA), or (VB), one of X₇, X₈, X₉, X₁₀, and X₁₁ is N.

In some embodiments of the compounds represented by formula (IIIA) or (IIIB), each R₃₁ is halo;

L is —NHC(O)—; R₁ is selected from the group consisting of halo or an optionally substituted lower alkyl; and R₃₀ is selected from the group consisting of —OR₅ and optionally substituted heteroaryl. In one aspect, each R₃₁ is F. In one aspect, X₁ and X₂ are both CH. In another aspect, X₁ and X₂ are both N. In a further aspect, X₁ is N and X₂ is CH.

In some embodiments of the compounds represented by formula (VA), or (VB), each R₃₁ is halo;

L is —NHC(O)—; R₁ is selected from the group consisting of halo or an optionally substituted lower alkyl; and two of X₇, X₈, X₉, X₁₀, and X₁₁ are N. In one aspect, each R₃₁ is F. In one aspect, X₁ and X₂ are both CH. In another aspect, X₁ and X₂ are both N. In a further aspect, X₁ is N and X₂ is CH.

In one embodiment, the compounds of the invention are not compounds disclosed in U.S. patent application Ser. No. 11/699,002.

In another embodiment, the invention relates to compounds selected from the group consisting of:

-   ethyl     3-(6-(2,6-difluorobenzamido)pyridin-3-yl)-4-methyl-5,6-dihydropyridine-1(2H)-carboxylate; -   allyl     3-(acetoxymethyl)-4-(4-(2,6-difluorobenzamido)phenyl)-5,6-dihydropyridine-1(2H)-carboxylate; -   2,6-difluoro-N-(5-(4-methyl-1-picolinoyl-1,2,5,6-tetrahydropyridin-3-yl)pyridin-2-yl)benzamide     hydrochloride; -   ethyl     3-(acetoxymethyl)-4-(4-(2,6-difluorobenzamido)phenyl)-5,6-dihydropyridine-1(2H)-carboxylate     or -   ethyl     4-chloro-3-(4-(2,6-difluorobenzamido)phenyl)-5,6-dihydropyridine-1(2H)-carboxylate;     -   or pharmaceutically acceptable salts, solvates, clathrates, or         prodrugs thereof.

In another embodiment, the invention relates to 2,6-difluoro-N-(5-(4-methyl-1-(pyrimidin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide hydrochloride or pharmaceutically acceptable salts, solvates, clathrates, or prodrugs thereof.

In another embodiment, the invention relates to

-   2,6-difluoro-N-(5-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyridin-2-yl)benzamide; -   2,6-difluoro-N-(5-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyridin-2-yl)benzamide     hydrochloride; -   2,6-difluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide; -   2,4-difluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide; -   2,6-difluoro-N-(5-(4-methyl-1-(pyrazin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide; -   N-(5-(1-(3-chloropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide; -   2,6-difluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide     hydrochloride; -   2-chloro-6-fluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide     hydrochloride; -   N-(5-(1-(3-chloropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide     hydrochloride; -   N-(5-(1-(3-chloropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,4-difluorobenzamide; -   2,6-difluoro-N-(5-(1-(3-fluoropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide     hydrochloride; -   2,4-difluoro-N-(5-(1-(3-fluoropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide     hydrochloride; -   N-(5-(1-(3-cyanopyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide; -   N-(5-(1-(3,5-difluoropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide; -   N-(5-(1-(3,5-dichloropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide; -   N-(5-(1-(3-bromopyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide; -   N-(4-(1-(3-aminopyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)phenyl)-2,6-difluorobenzamide; -   4-(1-(3-chloropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)-N-(2,6-difluorobenzyl)aniline; -   N-(4-(1-(3-cyanopyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)phenyl)-2,6-difluorobenzamide; -   2,6-difluoro-N-(4-(1-(3-fluoropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide; -   N-(4-(1-(3-chloropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)phenyl)-2,6-difluorobenzamide;     or

In another embodiment, the invention relates to

-   2,6-difluoro-N-(5-(4-methyl-1-(pyrimidin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide     hydrochloride -   2,6-difluoro-N-(5-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyridin-2-yl)benzamide; -   2,6-difluoro-N-(5-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyridin-2-yl)benzamide     hydrochloride; -   2,6-difluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide; -   2,4-difluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide; -   2,6-difluoro-N-(5-(4-methyl-1-(pyrazin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide; -   N-(5-(1-(3-chloropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide; -   2,6-difluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide     hydrochloride; -   2-chloro-6-fluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide     hydrochloride; -   N-(5-(1-(3-chloropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide     hydrochloride; -   N-(5-(1-(3-chloropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,4-difluorobenzamide; -   2,6-difluoro-N-(5-(1-(3-fluoropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide     hydrochloride; -   2,4-difluoro-N-(5-(1-(3-fluoropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide     hydrochloride; -   N-(5-(1-(3-cyanopyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide; -   N-(5-(1-(3,5-difluoropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide; -   N-(5-(1-(3,5-dichloropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide; -   N-(5-(1-(3-bromopyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide; -   N-(4-(1-(3-aminopyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)phenyl)-2,6-difluorobenzamide; -   4-(1-(3-chloropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)-N-(2,6-difluorobenzyl)aniline; -   N-(4-(1-(3-cyanopyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)phenyl)-2,6-difluorobenzamide; -   2,6-difluoro-N-(4-(1-(3-fluoropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide; -   N-(4-(1-(3-chloropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)phenyl)-2,6-difluorobenzamide;     or -   2,6-difluoro-N-(4-(4-methyl-1-(pyrazin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide;

or pharmaceutically acceptable salts, solvates, clathrates, or prodrugs thereof.

All of the features, specific embodiments and particular substituents disclosed herein may be combined in any combination. Each feature, embodiment or substituent disclosed in this specification may be replaced by an alternative feature, embodiment or substituent serving the same, equivalent, or similar purpose. In the case of chemical compounds, specific values for variables (e.g., values shown in the exemplary compounds disclosed herein) in any chemical formula disclosed herein can be combined in any combination resulting in a stable structure. Furthermore, specific values (whether preferred or not) for substituents in one type of chemical structure may be combined with values for other substituents (whether preferred or not) in the same or different type of chemical structure. Thus, unless expressly stated otherwise, each feature, embodiment or substituent disclosed is only an example of a generic series of equivalent or similar features, embodiments or substituents.

In another embodiment, the invention relates to pharmaceutical compositions that comprise a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof, as an active ingredient, and a pharmaceutically acceptable carrier or vehicle. The compositions are useful for immunosuppression or to treat or prevent inflammatory conditions, allergic conditions or immune disorders.

In another embodiment, the invention relates to methods for immunosuppression or for treating or preventing inflammatory conditions, immune disorders, or allergic disorders in a patient in need thereof comprising administering an effective amount of a compound represented by any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof.

In another embodiment, the invention relates to methods for immunosuppression or for treating or preventing inflammatory conditions, immune disorders, or allergic disorders in a patient in need thereof comprising administering an effective amount of a pharmaceutical composition that comprises a compound represented by any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof.

In another embodiment, compounds of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof, are particularly useful inhibiting immune cell (e.g., T-cells and/or B-cells) activation (e.g., activation in response to an antigen) and/or T cell and/or B cell proliferation. Indicators of immune cell activation include secretion of IL-2 by T cells, proliferation of T cells and/or B cells, and the like. In one embodiment, immune cell activation and/or T cell and/or B cell proliferation is inhibited in a mammal (e.g., a human), by administering to the mammal (e.g., human) a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof.

In another embodiment, compounds of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof, can inhibit the production of certain cytokines that regulate immune cell activation. For example, compounds of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof, can inhibit the production of IL-2, IL-4, IL-5, IL-13, GM-CSF, IFN-γ, TNF-α and combinations thereof. In one embodiment, cytokine production is inhibited in a mammal (e.g., a human), by administering to the mammal (e.g., human) a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof.

In another embodiment, compounds of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof, can modulate the activity of one or more ion channel, such as CRAC ion channels, involved in activation of immune cells. In one embodiment, a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1 can inhibit the influx of calcium ions into an immune cell (e.g., T cells, B cells, and/or mast cells) by inhibiting the action of CRAC ion channels either directly or indirectly. In general, a decrease in I_(CRAC) current upon contacting a cell with a compound is one indicator that the compound inhibitions CRAC ion channels. I_(CRAC) current can be measured, for example, using a patch clamp technique, which is described in more detail in the examples below. In one embodiment, a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1 modulates an ion channel in a mammal (e.g., a human). In one embodiment, the activity of one or more ion channels is inhibited in a mammal (e.g., a human), by administering to the mammal (e.g., human) a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof.

In another embodiment, compounds of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof, can inhibit degranulation of mast cell. Inhibition of mast cell degranulation can determined as described in the experimental section herein or by any method known to those skilled in the art. In one embodiment, mast cell degranulation is inhibited in a mammal (e.g., a human), by administering to the mammal (e.g., human) a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof.

EXEMPLARY COMPOUNDS OF THE INVENTION

Exemplary compounds of the invention are depicted in Table 1 below.

TABLE 1 Compound No. Structure Chemical Name 1

ethyl 3-(6-(2,6- difluorobenzamido)pyridin-3-yl)- 4-methyl-5,6-dihydropyridine- 1(2H)-carboxylate 2

2,6-difluoro-N-(5-(4-methyl-1- (pyrimidin-2-yl)-1,2,5,6- tetrahydropyridin-3-yl)pyrazin-2- yl)benzamide hydrochloride 3

allyl 3-(acetoxymethyl)-4-(4-(2,6- difluorobenzamido)phenyl)-5,6- dihydropyridine-1(2H)- carboxylate 4

2,6-difluoro-N-(5-(4-methyl-1- picolinoyl-1,2,5,6- tetrahydropyridin-3-yl)pyridin-2- yl)benzamide hydrochloride 5

ethyl 3-(acetoxymethyl)-4-(4-(2,6- difluorobenzamido)phenyl)-5,6- dihydropyridine-1(2H)- carboxylate 6

ethyl 4-chloro-3-(4-(2,6- difluorobenzamido)phenyl)-5,6- dihydropyridine-1(2H)- carboxylate 7

2,6-difluoro-N-(5-(4-methyl-1- (pyridin-2-yl)-1,2,5,6- tetrahydropyridin-3-yl)pyridin-2- yl)benzamide 8

2,6-difluoro-N-(5-(4-methyl-1- (pyridin-2-yl)-1,2,5,6- tetrahydropyridin-3-yl)pyridin-2- yl)benzamide hydrochloride 9

2,6-difluoro-N-(4-(4-methyl-1- (pyridin-2-yl)-1,2,5,6- tetrahydropyridin-3- yl)phenyl)benzamide 10

2,4-difluoro-N-(4-(4-methyl-1- (pyridin-2-yl)-1,2,5,6- tetrahydropyridin-3- yl)phenyl)benzamide 11

2,6-difluoro-N-(5-(4-methyl-1- (pyrazin-2-yl)-1,2,5,6- tetrahydropyridin-3-yl)pyrazin-2- yl)benzamide 12

N-(5-(1-(3-chloropyrazin-2-yl)-4- methyl-1,2,5,6-tetrahydropyridin- 3-yl)pyrazin-2-yl)-2,6- difluorobenzamide 13

2,6-difluoro-N-(4-(4-methyl-1- (pyridin-2-yl)-1,2,5,6- tetrahydropyridin-3- yl)phenyl)benzamide hydrochloride 14

2-chloro-6-fluoro-N-(4-(4-methyl- 1-(pyridin-2-yl)-1,2,5,6- tetrahydropyridin-3- yl)phenyl)benzamide hydrochloride 15

N-(5-(1-(3-chloropyridin-2-yl)-4- methyl-1,2,5,6-tetrahydropyridin- 3-yl)pyrazin-2-yl)-2,6- difluorobenzamide hydrochloride 16

N-(5-(1-(3-chloropyrazin-2-yl)-4- methyl-1,2,5,6-tetrahydropyridin- 3-yl)pyrazin-2-yl)-2,4- difluorobenzamide 17

2,6-difluoro-N-(5-(1-(3- fluoropyridin-2-yl)-4-methyl- 1,2,5,6-tetrahydropyridin-3- yl)pyrazin-2-yl)benzamide hydrochloride 18

2,4-difluoro-N-(5-(1-(3- fluoropyridin-2-yl)-4-methyl- 1,2,5,6-tetrahydropyridin-3- yl)pyrazin-2-yl)benzamide hydrochloride 19

N-(5-(1-(3-cyanopyridin-2-yl)-4- methyl-1,2,5,6-tetrahydropyridin- 3-yl)pyrazin-2-yl)-2,6- difluorobenzamide 20

N-(5-(1-(3,5-difluoropyridin-2-yl)- 4-methyl-1,2,5,6- tetrahydropyridin-3-yl)pyrazin-2- yl)-2,6-difluorobenzamide 21

N-(5-(1-(3,5-dichloropyridin-2-yl)- 4-methyl-1,2,5,6- tetrahydropyridin-3-yl)pyrazin-2- yl)-2,6-difluorobenzamide 22

N-(5-(1-(3-bromopyridin-2-yl)-4- methyl-1,2,5,6-tetrahydropyridin- 3-yl)pyrazin-2-yl)-2,6- difluorobenzamide 23

N-(4-(1-(3-aminopyrazin-2-yl)-4- methyl-1,2,5,6-tetrahydropyridin- 3-yl)phenyl)-2,6- difluorobenzamide 24

4-(1-(3-chloropyrazin-2-yl)-4- methyl-1,2,5,6-tetrahydropyridin- 3-yl)-N-(2,6- difluorobenzyl)aniline 25

N-(4-(1-(3-cyanopyrazin-2-yl)-4- methyl-1,2,5,6-tetrahydropyridin- 3-yl)phenyl)-2,6- difluorobenzamide 26

2,6-difluoro-N-(4-(1-(3- fluoropyrazin-2-yl)-4-methyl- 1,2,5,6-tetrahydropyridin-3- yl)phenyl)benzamide 27

N-(4-(1-(3-chloropyrazin-2-yl)-4- methyl-1,2,5,6-tetrahydropyridin- 3-yl)phenyl)-2,6- difluorobenzamide 28

2,6-difluoro-N-(4-(4-methyl-1- (pyrazin-2-yl)-1,2,5,6- tetrahydropyridin-3- yl)phenyl)benzamide

Mechanism of Action

Activation of T-lymphocytes in response to an antigen is dependent on calcium ion oscillations. Calcium ion oscillations in T-lymphocytes are triggered through stimulation of the T-cell antigen receptor, and involve calcium ion influx through the stored-operated Ca²⁺-release-activated Ca²⁺ (CRAC) channel. In addition, antigen induced degranulation of mast cells has also been shown to be initiated by calcium ion in flux. Although the molecular structure of the CRAC ion channel has not been identified, a detailed electrophysiological profile of the channel exist. Thus, modulation (e.g., inhibition) of CRAC ion channels can be measured by measuring modulation (e.g., inhibition) of the I_(CRAC) current. Calcium ion oscillations in T-cells have been implicated in the activation of several transcription factors (e.g., NFAT, Oct/Oap and NFκB) which are critical for T-cell activation (Lewis, Biochemical Society Transactions (2003), 31:925-929, the entire teachings of which are incorporated herein by reference). Without wishing to be bound by any theory, it is believed that because the compounds of the invention inhibit the activity of CRAC ion channels, they inhibit immune cell activation.

Methods of Treatment and Prevention

In accordance with the invention, an effective amount of a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, and prodrug thereof, or a pharmaceutical composition comprising a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, and prodrug thereof, is administered to a patient in need of immunosuppression or in need of treatment or prevention of an inflammatory condition, an immune disorder, or an allergic disorder. Such patients may be treatment naïve or may experience partial or no response to conventional therapies.

Responsiveness to immunosuppression or of a particular inflammatory condition, immune disorder, or allergic disorder in a subject can be measured directly (e.g., measuring blood levels of inflammatory cytokines (such as IL-2, IL-4, IL-5, IL-13, GM-CSF, TNF-α, IFN-γ and the like) after administration of a compound of this invention), or can be inferred based on an understanding of disease etiology and progression. The compounds of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or pharmaceutically acceptable salts, solvates, clathrates, and prodrugs thereof can be assayed in vitro or in vivo, for the desired therapeutic or prophylactic activity, prior to use in humans. For example, known animal models of inflammatory conditions, immune disorders, or allergic disorders can be used to demonstrate the safety and efficacy of compounds of this invention.

Preparation of Compounds of the Invention

General Coupling Procedure for Boronic Esters

To a solution of Pd(PhCN)₂Cl₂ (767 mg, 2 mmol) and 1,4-bis(diphenylphosphino)-butane (dppb) (1.28 g, 3 mmol) in 100 mL of toluene was added a boronic ester (XXXVII) (22.6 mmol) followed by an alkenyl halide or alkenyl triflate (XXXVI) (22.6 mmol), and sodium carbonate (3.4 g, 22.6 mmol), followed by 23 mL of water and 2 mL of methanol. This mixture was heated to 100° C. and refluxed for 18 hours. After allowing cooling to room temperature, the reaction was quenched with water and worked-up with ethyl acetate. The organic solution was then filtered through silica gel, evaporated in vacuo to give an amine derivative (XXXVIII) (see Scheme I) that was used without further purification in the next step.

General Procedure for Amide Coupling Reaction

Amine derivative (XXXVIII) (19.2 mmol) was dissolved in methylene chloride and cooled to 0° C. Pyridine (3.87 ml, 48 mmol) was added to the reaction mixture followed by an acyl chloride (XXXIX) (19.2 mmol). This mixture was allowed to warm to room temperature for 35 minutes at which time water was added and the reaction mixture was partitioned between the methylene chloride and water layers. The organic layer was collected and dried over a drying agent such as NaSO₄, then concentrated. The product was purified using flash chromatography to give a compound of the invention (XL) (see Scheme II).

Compounds of the invention in which L is —NHC(S)— or —C(S)NH— can be prepared by treating compounds having an amide linker with Lawesson's reagent.

Compounds of the invention having —CH₂—NH— or —NH—CH₂— linkers can be prepared by contacting compounds having —NHC(S)— or —C(S)NH— linkers with Raney Ni. Alternatively, compounds of the invention having a —CH₂—NH— or —NH—CH₂— linker can be prepared by reducing a compound having a —C(O)—NH— or —NH—C(O)— linker, respectively, with, for example, sodium borohydride (see U.S. patent application Ser. No. 10/897,681, filed on Jul. 22, 2004, the entire teachings of which are incorporated herein by reference).

Compounds of the invention having a thiourea linker (—NHC(S)NH—) can be prepared by treating compounds having a urea linker with Lawesson's reagent.

Compounds of the invention having an amide linker in which the amine group is attached to Y (—C(O)NH—) can be prepared by combining a halo-benzyl chloride or halo-pyridine carbonyl chloride (LII) with an amine derivative (XXVII) in pyridine to form a halo-amide intermediate (XXVIII). The reaction conditions are similar to those described for the amide coupling reaction above. A solution of Pd(PhCN)₂Cl₂ (767 mg, 2 mmol) and 1,4-bis(diphenylphosphino)-butane (dppb) (1.28 g, 3 mmol) in 100 mL of toluene is prepared. A cycloalkylene boronic acid ester (XXIX) is added to the solution, followed by the halo-amide intermediate (XXVIII) (22.6 mmol), and sodium carbonate (22.6 mmol), followed by 23 mL of water and 2 mL of methanol. This mixture was heated to 100° C. and refluxed for about 18 hours. After allowing cooling to room temperature, the reaction was quenched with water and worked-up with ethyl acetate to form a compound of the invention having a —C(O)NH— linker (XXX) (see Scheme VII).

Pharmaceutical Compositions and Dosage Forms

Pharmaceutical compositions and dosage forms of the invention comprise one or more active ingredients in relative amounts and formulated in such a way that a given pharmaceutical composition or dosage form can be used for immunosuppression or to treat or prevent inflammatory conditions, immune disorders, and allergic disorders. Preferred pharmaceutical compositions and dosage forms comprise a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable prodrug, salt, solvate, or clathrate thereof, optionally in combination with one or more additional active agents.

Single unit dosage forms of the invention are suitable for oral, mucosal (e.g., nasal, sublingual, vaginal, buccal, or rectal), parenteral (e.g., subcutaneous, intravenous, bolus injection, intramuscular, or intraarterial), or transdermal administration to a patient. Examples of dosage forms include, but are not limited to: tablets; caplets; capsules, such as soft elastic gelatin capsules; cachets; troches; lozenges; dispersions; suppositories; ointments; cataplasms (poultices); pastes; powders; dressings; creams; plasters; solutions; patches; aerosols (e.g., nasal sprays or inhalers); gels; liquid dosage forms suitable for oral or mucosal administration to a patient, including suspensions (e.g., aqueous or non-aqueous liquid suspensions, oil-in-water emulsions, or a water-in-oil liquid emulsions), solutions, and elixirs; liquid dosage forms suitable for parenteral administration to a patient; and sterile solids (e.g., crystalline or amorphous solids) that can be reconstituted to provide liquid dosage forms suitable for parenteral administration to a patient.

The composition, shape, and type of dosage forms of the invention will typically vary depending on their use. For example, a dosage form suitable for mucosal administration may contain a smaller amount of active ingredient(s) than an oral dosage form used to treat the same indication. This aspect of the invention will be readily apparent to those skilled in the art. See, e.g., Remington's Pharmaceutical Sciences (1990) 18th ed., Mack Publishing, Easton Pa.

Typical pharmaceutical compositions and dosage forms comprise one or more excipients. Suitable excipients are well known to those skilled in the art of pharmacy, and non-limiting examples of suitable excipients are provided herein. Whether a particular excipient is suitable for incorporation into a pharmaceutical composition or dosage form depends on a variety of factors well known in the art including, but not limited to, the way in which the dosage form will be administered to a patient. For example, oral dosage forms such as tablets may contain excipients not suited for use in parenteral dosage forms.

The suitability of a particular excipient may also depend on the specific active ingredients in the dosage form. For example, the decomposition of some active ingredients can be accelerated by some excipients such as lactose, or when exposed to water. Active ingredients that comprise primary or secondary amines (e.g., N-desmethylvenlafaxine and N,N-didesmethylvenlafaxine) are particularly susceptible to such accelerated decomposition. Consequently, this invention encompasses pharmaceutical compositions and dosage forms that contain little, if any, lactose. As used herein, the term “lactose-free” means that the amount of lactose present, if any, is insufficient to substantially increase the degradation rate of an active ingredient. Lactose-free compositions of the invention can comprise excipients that are well known in the art and are listed, for example, in the U.S. Pharmocopia (USP) SP (XXI)/NF (XVI). In general, lactose-free compositions comprise active ingredients, a binder/filler, and a lubricant in pharmaceutically compatible and pharmaceutically acceptable amounts. Preferred lactose-free dosage forms comprise active ingredients, microcrystalline cellulose, pre-gelatinized starch, and magnesium stearate.

This invention further encompasses anhydrous pharmaceutical compositions and dosage forms comprising active ingredients, since water can facilitate the degradation of some compounds. For example, the addition of water (e.g., 5%) is widely accepted in the pharmaceutical arts as a means of simulating long-term storage in order to determine characteristics such as shelf-life or the stability of formulations over time. See, e.g., Jens T. Carstensen (1995) Drug Stability: Principles & Practice, 2d. Ed., Marcel Dekker, NY, N.Y., 379-80. In effect, water and heat accelerate the decomposition of some compounds. Thus, the effect of water on a formulation can be of great significance since moisture and/or humidity are commonly encountered during manufacture, handling, packaging, storage, shipment, and use of formulations.

Anhydrous pharmaceutical compositions and dosage forms of the invention can be prepared using anhydrous or low moisture containing ingredients and low moisture or low humidity conditions. Pharmaceutical compositions and dosage forms that comprise lactose and at least one active ingredient that comprises a primary or secondary amine are preferably anhydrous if substantial contact with moisture and/or humidity during manufacturing, packaging, and/or storage is expected.

An anhydrous pharmaceutical composition should be prepared and stored such that its anhydrous nature is maintained. Accordingly, anhydrous compositions are preferably packaged using materials known to prevent exposure to water such that they can be included in suitable formulary kits. Examples of suitable packaging include, but are not limited to, hermetically sealed foils, plastics, unit dose containers (e.g., vials), blister packs, and strip packs.

The invention further encompasses pharmaceutical compositions and dosage forms that comprise one or more compounds that reduce the rate by which an active ingredient will decompose. Such compounds, which are referred to herein as “stabilizer” include, but are not limited to, antioxidants such as ascorbic acid, pH buffers, or salt buffers.

Like the amounts and types of excipients, the amounts and specific types of active ingredients in a dosage form may differ depending on factors such as, but not limited to, the route by which it is to be administered to patients. However, typical dosage forms of the invention comprise a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof in an amount of from about 1 mg to about 1000 mg, preferably in an amount of from about 50 mg to about 500 mg, and most preferably in an amount of from about 75 mg to about 350 mg. The typical total daily dosage of a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof can range from about 1 mg to about 5000 mg per day, preferably in an amount from about 50 mg to about 1500 mg per day, more preferably from about 75 mg to about 1000 mg per day. It is within the skill of the art to determine the appropriate dose and dosage form for a given patient.

Oral Dosage Forms

Pharmaceutical compositions of the invention that are suitable for oral administration can be presented as discrete dosage forms, such as, but are not limited to, tablets (e.g., chewable tablets), caplets, capsules, and liquids (e.g., flavored syrups). Such dosage forms contain predetermined amounts of active ingredients, and may be prepared by methods of pharmacy well known to those skilled in the art. See generally, Remington's Pharmaceutical Sciences (1990) 18th ed., Mack Publishing, Easton Pa.

Typical oral dosage forms of the invention are prepared by combining the active ingredient(s) in an admixture with at least one excipient according to conventional pharmaceutical compounding techniques. Excipients can take a wide variety of forms depending on the form of preparation desired for administration. For example, excipients suitable for use in oral liquid or aerosol dosage forms include, but are not limited to, water, glycols, oils, alcohols, flavoring agents, preservatives, and coloring agents. Examples of excipients suitable for use in solid oral dosage forms (e.g., powders, tablets, capsules, and caplets) include, but are not limited to, starches, sugars, micro-crystalline cellulose, diluents, granulating agents, lubricants, binders, and disintegrating agents.

Because of their ease of administration, tablets and capsules represent the most advantageous oral dosage unit forms, in which case solid excipients are employed. If desired, tablets can be coated by standard aqueous or nonaqueous techniques. Such dosage forms can be prepared by any of the methods of pharmacy. In general, pharmaceutical compositions and dosage forms are prepared by uniformly and intimately admixing the active ingredients with liquid carriers, finely divided solid carriers, or both, and then shaping the product into the desired presentation if necessary.

For example, a tablet can be prepared by compression or molding. Compressed tablets can be prepared by compressing in a suitable machine the active ingredients in a free-flowing form such as powder or granules, optionally mixed with an excipient. Molded tablets can be made by molding in a suitable machine a mixture of the powdered compound moistened with an inert liquid diluent.

Examples of excipients that can be used in oral dosage forms of the invention include, but are not limited to, binders, fillers, disintegrants, and lubricants. Binders suitable for use in pharmaceutical compositions and dosage forms include, but are not limited to, corn starch, potato starch, or other starches, gelatin, natural and synthetic gums such as acacia, sodium alginate, alginic acid, other alginates, powdered tragacanth, guar gum, cellulose and its derivatives (e.g., ethyl cellulose, cellulose acetate, carboxymethyl cellulose calcium, sodium carboxymethyl cellulose), polyvinyl pyrrolidone, methyl cellulose, pre-gelatinized starch, hydroxypropyl methyl cellulose, (e.g., Nos. 2208, 2906, 2910), microcrystalline cellulose, and mixtures thereof.

Suitable forms of microcrystalline cellulose include, but are not limited to, the materials sold as AVICEL-PH-101, AVICEL-PH-103 AVICEL RC-581, AVICEL-PH-105 (available from FMC Corporation, American Viscose Division, Avicel Sales, Marcus Hook, Pa.), and mixtures thereof. One specific binder is a mixture of microcrystalline cellulose and sodium carboxymethyl cellulose sold as AVICEL RC-581. Suitable anhydrous or low moisture excipients or additives include AVICEL-PH-103J and Starch 1500 LM.

Examples of fillers suitable for use in the pharmaceutical compositions and dosage forms disclosed herein include, but are not limited to, talc, calcium carbonate (e.g., granules or powder), microcrystalline cellulose, powdered cellulose, dextrates, kaolin, mannitol, silicic acid, sorbitol, starch, pre-gelatinized starch, and mixtures thereof. The binder or filler in pharmaceutical compositions of the invention is typically present in from about 50 to about 99 weight percent of the pharmaceutical composition or dosage form.

Disintegrants are used in the compositions of the invention to provide tablets that disintegrate when exposed to an aqueous environment. Tablets that contain too much disintegrant may disintegrate in storage, while those that contain too little may not disintegrate at a desired rate or under the desired conditions. Thus, a sufficient amount of disintegrant that is neither too much nor too little to detrimentally alter the release of the active ingredients should be used to form solid oral dosage forms of the invention. The amount of disintegrant used varies based upon the type of formulation, and is readily discernible to those of ordinary skill in the art. Typical pharmaceutical compositions comprise from about 0.5 to about 15 weight percent of disintegrant, preferably from about 1 to about 5 weight percent of disintegrant.

Disintegrants that can be used in pharmaceutical compositions and dosage forms of the invention include, but are not limited to, agar-agar, alginic acid, calcium carbonate, microcrystalline cellulose, croscarmellose sodium, crospovidone, polacrilin potassium, sodium starch glycolate, potato or tapioca starch, other starches, pre-gelatinized starch, other starches, clays, other algins, other celluloses, gums, and mixtures thereof.

Lubricants that can be used in pharmaceutical compositions and dosage forms of the invention include, but are not limited to, calcium stearate, magnesium stearate, mineral oil, light mineral oil, glycerin, sorbitol, mannitol, polyethylene glycol, other glycols, stearic acid, sodium lauryl sulfate, talc, hydrogenated vegetable oil (e.g., peanut oil, cottonseed oil, sunflower oil, sesame oil, olive oil, corn oil, and soybean oil), zinc stearate, ethyl oleate, ethyl laureate, agar, and mixtures thereof. Additional lubricants include, for example, a syloid silica gel (AEROSIL 200, manufactured by W.R. Grace Co. of Baltimore, Md.), a coagulated aerosol of synthetic silica (marketed by Degussa Co. of Plano, Tex.), CAB-O-SIL (a pyrogenic silicon dioxide product sold by Cabot Co. of Boston, Mass.), and mixtures thereof. If used at all, lubricants are typically used in an amount of less than about 1 weight percent of the pharmaceutical compositions or dosage forms into which they are incorporated.

Controlled Release Dosage Forms

Active ingredients of the invention can be administered by controlled release means or by delivery devices that are well known to those of ordinary skill in the art. Examples include, but are not limited to, those described in U.S. Pat. Nos. 3,845,770; 3,916,899; 3,536,809; 3,598,123; and 4,008,719, 5,674,533, 5,059,595, 5,591,767, 5,120,548, 5,073,543, 5,639,476, 5,354,556, and 5,733,566, each of which is incorporated herein by reference. Such dosage forms can be used to provide slow or controlled-release of one or more active ingredients using, for example, hydropropylmethyl cellulose, other polymer matrices, gels, permeable membranes, osmotic systems, multilayer coatings, microparticles, liposomes, microspheres, or a combination thereof to provide the desired release profile in varying proportions. Suitable controlled-release formulations known to those of ordinary skill in the art, including those described herein, can be readily selected for use with the active ingredients of the invention. The invention thus encompasses single unit dosage forms suitable for oral administration such as, but not limited to, tablets, capsules, gelcaps, and caplets that are adapted for controlled-release.

All controlled-release pharmaceutical products have a common goal of improving drug therapy over that achieved by their non-controlled counterparts. Ideally, the use of an optimally designed controlled-release preparation in medical treatment is characterized by a minimum of drug substance being employed to cure or control the condition in a minimum amount of time. Advantages of controlled-release formulations include extended activity of the drug, reduced dosage frequency, and increased patient compliance. In addition, controlled-release formulations can be used to affect the time of onset of action or other characteristics, such as blood levels of the drug, and can thus affect the occurrence of side (e.g., adverse) effects.

Most controlled-release formulations are designed to initially release an amount of drug (active ingredient) that promptly produces the desired therapeutic effect, and gradually and continually release of other amounts of drug to maintain this level of therapeutic or prophylactic effect over an extended period of time. In order to maintain this constant level of drug in the body, the drug must be released from the dosage form at a rate that will replace the amount of drug being metabolized and excreted from the body. Controlled-release of an active ingredient can be stimulated by various conditions including, but not limited to, pH, temperature, enzymes, water, or other physiological conditions or compounds.

A particular extended release formulation of this invention comprises a therapeutically or prophylactically effective amount of a compound of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1, or a pharmaceutically acceptable salt, solvate, hydrate, clathrate, or prodrug thereof, in spheroids which further comprise microcrystalline cellulose and, optionally, hydroxypropylmethyl-cellulose coated with a mixture of ethyl cellulose and hydroxypropylmethylcellulose. Such extended release formulations can be prepared according to U.S. Pat. No. 6,274,171, the entire teachings of which are incorporated herein by reference.

A specific controlled-release formulation of this invention comprises from about 6% to about 40% a compound of any one of formulas (I), (IIA), (IIB), (IIIA), (IIIB), (IVA), (IVB), (VA), or (VB), or Table 1 by weight, about 50% to about 94% microcrystalline cellulose, NF, by weight, and optionally from about 0.25% to about 1% by weight of hydroxypropyl-methylcellulose, USP, wherein the spheroids are coated with a film coating composition comprised of ethyl cellulose and hydroxypropylmethylcellulose.

Parenteral Dosage Forms

Parenteral dosage forms can be administered to patients by various routes including, but not limited to, subcutaneous, intravenous (including bolus injection), intramuscular, and intraarterial. Because their administration typically bypasses patients' natural defenses against contaminants, parenteral dosage forms are preferably sterile or capable of being sterilized prior to administration to a patient. Examples of parenteral dosage forms include, but are not limited to, solutions ready for injection, dry products ready to be dissolved or suspended in a pharmaceutically acceptable vehicle for injection, suspensions ready for injection, and emulsions.

Suitable vehicles that can be used to provide parenteral dosage forms of the invention are well known to those skilled in the art. Examples include, but are not limited to: Water for Injection USP; aqueous vehicles such as, but not limited to, Sodium Chloride Injection, Ringer's Injection, Dextrose Injection, Dextrose and Sodium Chloride Injection, and Lactated Ringer's Injection; water-miscible vehicles such as, but not limited to, ethyl alcohol, polyethylene glycol, and polypropylene glycol; and non-aqueous vehicles such as, but not limited to, corn oil, cottonseed oil, peanut oil, sesame oil, ethyl oleate, isopropyl myristate, and benzyl benzoate.

Compounds that increase the solubility of one or more of the active ingredients disclosed herein can also be incorporated into the parenteral dosage forms of the invention.

Transdermal, Topical, and Mucosal Dosage Forms

Transdermal, topical, and mucosal dosage forms of the invention include, but are not limited to, ophthalmic solutions, sprays, aerosols, creams, lotions, ointments, gels, solutions, emulsions, suspensions, or other forms known to one of skill in the art. See, e.g., Remington's Pharmaceutical Sciences (1980 & 1990) 16th and 18th eds., Mack Publishing, Easton Pa. and Introduction to Pharmaceutical Dosage Forms (1985) 4th ed., Lea & Febiger, Philadelphia. Dosage forms suitable for treating mucosal tissues within the oral cavity can be formulated as mouthwashes or as oral gels. Further, transdermal dosage forms include “reservoir type” or “matrix type” patches, which can be applied to the skin and worn for a specific period of time to permit the penetration of a desired amount of active ingredients.

Suitable excipients (e.g., carriers and diluents) and other materials that can be used to provide transdermal, topical, and mucosal dosage forms encompassed by this invention are well known to those skilled in the pharmaceutical arts, and depend on the particular tissue to which a given pharmaceutical composition or dosage form will be applied. With that fact in mind, typical excipients include, but are not limited to, water, acetone, ethanol, ethylene glycol, propylene glycol, butane-1,3-diol, isopropyl myristate, isopropyl palmitate, mineral oil, and mixtures thereof to form lotions, tinctures, creams, emulsions, gels or ointments, which are non-toxic and pharmaceutically acceptable. Moisturizers or humectants can also be added to pharmaceutical compositions and dosage forms if desired. Examples of such additional ingredients are well known in the art. See, e.g., Remington's Pharmaceutical Sciences (1980 & 1990) 16th and 18th eds., Mack Publishing, Easton Pa.

Depending on the specific tissue to be treated, additional components may be used prior to, in conjunction with, or subsequent to treatment with active ingredients of the invention. For example, penetration enhancers can be used to assist in delivering the active ingredients to the tissue. Suitable penetration enhancers include, but are not limited to: acetone; various alcohols such as ethanol, oleyl, and tetrahydrofuryl; alkyl sulfoxides such as dimethyl sulfoxide; dimethyl acetamide; dimethyl formamide; polyethylene glycol; pyrrolidones such as polyvinylpyrrolidone; Kollidon grades (Povidone, Polyvidone); urea; and various water-soluble or insoluble sugar esters such as Tween 80 (polysorbate 80) and Span 60 (sorbitan monostearate).

The pH of a pharmaceutical composition or dosage form, or of the tissue to which the pharmaceutical composition or dosage form is applied, may also be adjusted to improve delivery of one or more active ingredients. Similarly, the polarity of a solvent carrier, its ionic strength, or tonicity can be adjusted to improve delivery. Compounds such as stearates can also be added to pharmaceutical compositions or dosage forms to advantageously alter the hydrophilicity or lipophilicity of one or more active ingredients so as to improve delivery. In this regard, stearates can serve as a lipid vehicle for the formulation, as an emulsifying agent or surfactant, and as a delivery-enhancing or penetration-enhancing agent. Different salts, hydrates or solvates of the active ingredients can be used to further adjust the properties of the resulting composition.

Combination Therapy

The methods for immunosuppression or for treating or preventing inflammatory conditions, allergic disorders, and immune disorders in a patient in need thereof can further comprise administering to the patient being administered a compound of this invention, an effective amount of one or more other active agents. Such active agents may include those used conventionally for immunosuppression or for inflammatory conditions, allergic disorders, or immune disorders. These other active agents may also be those that provide other benefits when administered in combination with the compounds of this invention. For example, other therapeutic agents may include, without limitation, steroids, non-steroidal anti-inflammatory agents, antihistamines, analgesics, immunosuppressive agents and suitable mixtures thereof. In such combination therapy treatment, both the compounds of this invention and the other drug agent(s) are administered to a subject (e.g., humans, male or female) by conventional methods. The agents may be administered in a single dosage form or in separate dosage forms. Effective amounts of the other therapeutic agents and dosage forms are well known to those skilled in the art. It is well within the skilled artisan's purview to determine the other therapeutic agent's optimal effective-amount range.

In one embodiment of the invention where another therapeutic agent is administered to a subject, the effective amount of the compound of this invention is less than its effective amount when the other therapeutic agent is not administered. In another embodiment, the effective amount of the conventional agent is less than its effective amount when the compound of this invention is not administered. In this way, undesired side effects associated with high doses of either agent may be minimized. Other potential advantages (including without limitation improved dosing regimens and/or reduced drug cost) will be apparent to those of skill in the art.

In one embodiment relating to autoimmune, allergic and inflammatory conditions, the other therapeutic agent may be a steroid or a non-steroidal anti-inflammatory agent. Particularly useful non-steroidal anti-inflammatory agents, include, but are not limited to, aspirin, ibuprofen, diclofenac, naproxen, benoxaprofen, flurbiprofen, fenoprofen, flubufen, ketoprofen, indoprofen, piroprofen, carprofen, oxaprozin, pramoprofen, muroprofen, trioxaprofen, suprofen, aminoprofen, tiaprofenic acid, fluprofen, bucloxic acid, indomethacin, sulindac, tolmetin, zomepirac, tiopinac, zidometacin, acemetacin, fentiazac, clidanac, oxpinac, mefenamic acid, meclofenamic acid, flufenamic acid, niflumic acid, tolfenamic acid, diflurisal, flufenisal, piroxicam, sudoxicam, isoxicam; salicylic acid derivatives, including aspirin, sodium salicylate, choline magnesium trisalicylate, salsalate, diflunisal, salicylsalicylic acid, sulfasalazine, and olsalazin; para-aminophennol derivatives including acetaminophen and phenacetin; indole and indene acetic acids, including indomethacin, sulindac, and etodolac; heteroaryl acetic acids, including tolmetin, diclofenac, and ketorolac; anthranilic acids (fenamates), including mefenamic acid, and meclofenamic acid; enolic acids, including oxicams (piroxicam, tenoxicam), and pyrazolidinediones (phenylbutazone, oxyphenthartazone); and alkanones, including nabumetone and pharmaceutically acceptable salts thereof and mixtures thereof. For a more detailed description of the NSAIDs, see Paul A. Inset, Analgesic-Antipyretic and Antiinflammatory Agents and Drugs Employed in the Treatment of Gout, in Goodman & Gilman's The Pharmacological Basis of Therapeutics 617-57 (Perry B. Molinhoff and Raymond W. Ruddon eds., 9^(th) ed 1996) and Glen R. Hanson, Analgesic, Antipyretic and Anti-Inflammatory Drugs in Remington: The Science and Practice of Pharmacy Vol II 1196-1221 (A. R. Gennaro ed. 19th ed. 1995) which are hereby incorporated by reference in their entireties.

Of particular relevance to allergic disorders, the other therapeutic agent may be an antihistamine. Useful antihistamines include, but are not limited to, loratadine, cetirizine, fexofenadine, desloratadine, diphenhydramine, chlorpheniramine, chlorcyclizine, pyrilamine, promethazine, terfenadine, doxepin, carbinoxamine, clemastine, tripelennamine, brompheniramine, hydroxyzine, cyclizine, meclizine, cyproheptadine, phenindamine, acrivastine, azelastine, levocabastine, and mixtures thereof. For a more detailed description of antihistamines, see Goodman & Gilman's The Pharmacological Basis of Therapeutics (2001) 651-57, 10^(th) ed).

Immunosuppressive agents include glucocorticoids, corticosteroids (such as Prednisone or Solumedrol), T cell blockers (such as cyclosporin A and FK506), purine analogs (such as azathioprine (Imuran)), pyrimidine analogs (such as cytosine arabinoside), alkylating agents (such as nitrogen mustard, phenylalanine mustard, buslfan, and cyclophosphamide), folic acid antagonsists (such as aminopterin and methotrexate), antibiotics (such as rapamycin, actinomycin D, mitomycin C, puramycin, and chloramphenicol), human IgG, antilymphocyte globulin (ALG), and antibodies (such as anti-CD3 (OKT3), anti-CD4 (OKT4), anti-CD5, anti-CD7, anti-IL-2 receptor, anti-alpha/beta TCR, anti-ICAM-1, anti-CD20 (Rituxan), anti-IL-12 and antibodies to immunotoxins).

The foregoing and other useful combination therapies will be understood and appreciated by those of skill in the art. Potential advantages of such combination therapies include a different efficacy profile, the ability to use less of each of the individual active ingredients to minimize toxic side effects, synergistic improvements in efficacy, improved ease of administration or use and/or reduced overall expense of compound preparation or formulation.

Other Embodiments

The compounds of this invention may be used as research tools (for example, as a positive control for evaluating other potential CRAC inhibitors, or IL-2, IL-4, IL-5, IL-13, GM-CSF, TNF-α, and/or INF-γ inhibitors). These and other uses and embodiments of the compounds and compositions of this invention will be apparent to those of ordinary skill in the art.

The invention is further defined by reference to the following examples describing in detail the preparation of compounds of the invention. It will be apparent to those skilled in the art that many modifications, both to materials and methods, may be practiced without departing from the purpose and interest of this invention. The following examples are set forth to assist in understanding the invention and should not be construed as specifically limiting the invention described and claimed herein. Such variations of the invention, including the substitution of all equivalents now known or later developed, which would be within the purview of those skilled in the art, and changes in formulation or minor changes in experimental design, are to be considered to fall within the scope of the invention incorporated herein.

EXAMPLES Experimental Rationale

Without wishing to be bound by theory, it is believed that the compounds of this invention inhibit CRAC ion channels, thereby inhibiting production of IL-2 and other key cytokines involved with inflammatory, allergic and immune responses. The examples that follow demonstrate these properties.

Materials and General Methods

Reagents and solvents used below can be obtained from commercial sources such as Aldrich Chemical Co. (Milwaukee, Wis., USA). ¹H-NMR and ¹³C-NMR spectra were recorded on a Varian 300 MHz NMR spectrometer. Significant peaks are tabulated in the order: δ (ppm): chemical shift, multiplicity (s, singlet; d, doublet; t, triplet; q, quartet; m, multiplet; br s, broad singlet), coupling constant(s) in Hertz (Hz) and number of protons.

Patch clamp experiments were performed in the tight-seal whole-cell configuration at 21-25° C. High resolution current recordings were acquired by a computer-based patch clamp amplifier system (EPC-9, HEKA, Lambrecht, Germany). Patch pipettes had resistances between 2-4 MΩ after filling with the standard intracellular solution. Immediately following establishment of the whole-cell configuration, voltage ramps of 50-200 ms duration spanning the voltage range of −100 to +100 mV were delivered at a rate of 0.5 Hz over a period of 300-400 seconds. All voltages were corrected for a liquid junction potential of 10 mV between external and internal solutions when using glutamate as the intracellular anion. Currents were filtered at 2.9 kHz and digitized at 10 μs intervals. Capacitive currents and series resistance were determined and corrected before each voltage ramp using the automatic capacitance compensation of the EPC-9. The low resolution temporal development of membrane currents was assessed by extracting the current amplitude at −80 mV or +80 mV from individual ramp current records.

Synthesis of Representative Exemplary Compounds of this Invention

In general, the compounds of the invention can be synthesized using methods analogous to those described in U.S. patent application Ser. No. 10/897,681 and U.S. patent application Ser. No. 11/233,224, the entire teachings of these patent applications are incorporated herein by reference.

General Suzuki Coupling Procedure for Boronic Esters:

To a solution of Pd(PhCN)₂Cl₂ (767 mg, 2 mmol) and 1,4-bis(diphenylphosphino)-butane (dppb) (1.28 g, 3 mmol) in 100 mL of toluene was added a boronic ester (XXXVII) (22.6 mmol) followed by an alkenyl halide or alkenyl triflate (XXXVI) (22.6 mmol), and sodium carbonate (3.4 g, 22.6 mmol), followed by 23 mL of water and 2 mL of methanol. This mixture was heated to 100° C. and refluxed for 18 hours. After allowing cooling to room temperature, the reaction was quenched with water and worked-up with ethyl acetate. The organic solution was then filtered through silica gel, evaporated in vacuo to give an amine derivative (XXXVIII) that was used without further purification in the next step.

General Procedure for Amide Formation Using Acyl Chloride:

An amine derivative (XXXVIII) (19.2 mmol) was dissolved in methylene chloride and cooled to 0° C. Pyridine (3.87 ml, 48 mmol) was added to the reaction mixture followed by an acyl chloride (XXXIX) (19.2 mmol). This mixture was allowed to warm to room temperature for 35 minutes at which time water was added and the reaction mixture was partitioned between the methylene chloride and water layers. The organic layer was collected and dried over a drying agent such as NaSO₃, then concentrated. The product was purified using flash chromatography to give a compound of the invention (XL).

General Procedure for Pyridinium Salt Formation

A solution of pyridine (200 mg, 0.62 mmol) was dissolved in dichloromethane (1.5 ml). Benzyl bromide (3 eq) was added and the room temperature solution was stirred for 24 hours. The solid was filtered and washed with cold dichloromethane to give pure pyridinium salt.

General Procedure for Reduction of Pyridinium Salt

The pyridinium salt (crude) was dissolved in methanol and sodium borohydride (113 mg, 3 eq) was added. This solution was warmed to 35 degrees for 5 hours then cooled to room temperature. The methanol was removed, water and methylene chloride added, and extracted. The 150 mg (70% yield) of tetrahydropyridine was produced after column chromatography.

General Procedure for Carbamate.

The benzyl amine (31 mg, 0.074 mmol) was dissolved in methylene chloride (2 ml) at room temperature. Ethyl chloroformate (14 ul, 2 eq) was added to the solution and it was stirred for 3 hours The solvent was removed and the crude mixture was flashed with ethyl acetate/hexane to produce 28.4 mg (97% yield) of carbamate.

General Procedure for Tetrahydropyridine Deprotection

10% Palladium on carbon (10 mg, 10% w/w) was added to a methanolic solution (1.5 ml) of benzylcarbamate (109 mg, 0.247 mmol). A ballon of hydrogen was added to the top of the flask and thoroughly stirred for 1 hour. After filtration completely pure free amine was recovered (73 mg, 96% yield). No further purification required.

General Procedure for Thiazole Amine Formation.

The amine (725 mg, 1.73 mmol) was dissolved in methylene chloride (5 ml) and cyanogen bromide (549 mg, 5.19 mmol) was added. This reaction was stirred at room temperature for 1 hour then purified by flash chromatography. Pure cyanamide (415 mg, 68% yield) was isolated as a white solid.

The cyanamide (55 mg, 0.01556 mmol) was dissolved in ethanol/ammonia (2 ml, 2M). hydrogen sulfide gas was then bubbled through the reaction solution for 20 minutes at which time the bubbling was siesed and the reaction was capped and stirred for 5 hours at room temperature. After completion was solvent was removed and the thiourea was recrystallized from ethanol to remove yellow color.

The thiourea was then dissolved in anhydrous THF (5 ml) and the bromide (140 mg, 0.709 mmol) was added and refluxed for 5 hours. The r×n mixture was then quenched with sodium bicarbonate solution and diluted with ethylacetate. After drying over magnesium sulfate, the compound was purified by flash chromotagraphy yielding 39 mg of pure thiazole product. The compound was then dissolved in ethanol and excess HCl in ether was added to form the salt. Solid precipitated out of solution at −40° C. and was filtered.

Example 1 Ethyl 3-(4-(2,6-difluorobenzamido)phenyl)-4-methyl-5,6-dihydropyridine-1(2H)-carboxylate

Compound A (31 mg, 0.074 mmol) was dissolved in methylene chloride (2 mL) at room temperature. Ethyl chloroformate (14 μl, 2 eq) was added to the solution and it was heated to 110° C. in the microwave for 1 hour. The solvent was removed and the crude mixture was flashed with ethyl acetate/hexane to produce 28.4 mg (97% yield) of Compound 12.

¹H NMR (300 MHz, CDCl₃) δ 7.65 (d, J=8.1 Hz, 2H), 7.36-7.48 (m, 1H), 7.19 (d, J=8.1 Hz, 2H), 6.997 (t, J=8.1 Hz, 1H), 4.00-4.20 (m, 3H), 3.59-3.63 (m, 1H), 2.17-2.20 (m, 3H), 1.63 (s, 3H), 1.26 (t, J=6.3 Hz, 3H) ppm.

ESMS calcd. (C22H22F2N2O3): 400.2; found: 401.2. (M+H).

Example 2 methyl 3-(4-(2,6-difluorobenzamido)phenyl)-4-(thiazol-2-yl)-5,6-dihydropyridine-1(2H)-carboxylate (B) and methyl 3-(4-(2,6-difluorobenzamido)phenyl)-4-isopropyl-5,6-dihydropyridine-1(2H)-carboxylate (C)

N-(4-(4-bromopyridin-3-yl)phenyl)-2,6-difluorobenzamide (e)

To a solution of 4-Bromopyridine hydrochloride (500 mg, 2.57 mmol) in THF (15 mL) was added LDA (2.0 N, 2.6 mL, 5.2 mmol) at −78° C. After 30 min, the solution of ZnCl₂ (1 M in ether, 3.1 mL, 3.1 mmol) in THF (5 mL) was added slowly. The cold bath was removed and the solution was allowed to warm up room temperature. To this solution was added Pd(PPh₃)₄ (50 mg) and compound d (370 mg, 1.03 mmol). The solution was heated at 65° C. for 2 hrs before it was diluted with EtOAc (20 mL) and H₂O (10 mL). The organic phase was collected, dried and concentrated. Column chromatography afforded Compound e (210 mg, 21%). NMR (300 MHz, (CD₃)₂SO) δ 10.99 (s, 1H), 8.52 (s, 1H), 8.40 (d, J=5.4 Hz, 1H), 7.84-7.78 (m, 3H), 7.63-7.57 (m, 1H), 7.47 (d, J=8.7 Hz, 2H), 7.27 (t, J=8.1 Hz, 2H); ESMS cacld (C₁₈H₁₁BrF₂N₂O): 388.0; found: 389.1 (M+H).

Example 3 2,6-Difluoro-N-[4-(1-methoxycarbonyl-4-thiazol-2-yl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide

The solution of Compound e (15 mg, 0.04 mmol), 2-thiazolylzinc bromide solution (0.5 M, 0.2 mL, 0.1 mmol), and Pd(PPh₃)₂Cl₂ (5 mg) in THF (4 mL) was heated in microwave synthesizer at 150° C. for 30 min. The solution was concentrated and chromatographied to give Compound f (13 mg, 83%). To the solution of Compound f (13 mg, 0.033 mmol) in MeOH (2 mL) was added benzyl bromide (BnBr) (0.05 mL, 0.4 mmol). After stirring 60 min, the solvent was removed and the residue was washed with EtOAc (5 mL) to remove BnBr. The resulting residue was dissolved in MeOH (4 mL) and added NaBH₄ (10 mg, 0.26 mmol). The solution was passed through a short column of silica gel. The filtrate was concentrated and dissolved in DCM (4 mL). After adding methyl chloroformate (0.1 mL), the solution was stirred overnight before it was concentrated and chromatographied to give Compound 41 (10 mg, 67%). ¹H NMR (300 MHz, CDCl₃) δ 7.79-7.62 (m, 4H), 7.52-7.38 (m, 1H), 7.28-7.18 (m, 2H), 7.09-6.98 (m, 3H), 4.26-4.18 (m, 2H), 3.74 (s, 3H), 2.95-2.88 (m, 2H), 1.14-1.09 (m, 2H); ESMS cacld (C₂₃H₁₉F₂N₃O₃S): 455.1; found: 456.2 (M+H).

Example 4 2,6-Difluoro-N-[4-(1-methoxycarbonyl-4-isopropyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide

To a solution of isopropylmagnesium chloride (2 M, 0.65 mL, 1.3 mmol) in Et₂O (5 mL) was added ZnCl₂ (1 M, 1.5 mL, 1.5 mmol) at 0° C. After 30 min, Compound e (50 mg, 0.13 mmol) was added. The solution was stirred at room temperature for 2 hrs before it was quenched with NH₄Cl (5 mL). The mixture was diluted with EtOAc and the organic phase was collected, dried, and concentrated. Column chromatography afforded Compound g (25 mg, 56%). From Compound g, Compound 42 was prepared following the same procedure as described for Compound 41 to give a 35% yield. ¹H NMR (300 MHz, CDCl₃) δ 7.64-7.59 (m, 3H), 7.45-7.38 (m, 1H), 7.19-7.16 (m, 2H), 7.14-6.97 (m, 2H), 4.06-3.99 (m, 2H), 3.73 (s, 3H), 3.62-3.58 (m, 2H), 2.22-2.18 (m, 2H), 1.94-1.89 (m, 1H), 1.57 (s, 6H); ESMS cacld (C₂₃H₂₄F₂N₂O₃): 414.2; found: 415.3 (M+H).

General Procedure for Pyridinium Salt Formation:

A solution of pyridine (200 mg, 0.62 mmol) was dissolved in dichloromethane (1.5 ml). Benzyl bromide (3 eq) was added and the room temperature solution was stirred for 24 hours. The solid was filtered and washed with cold dichloromethane to give pure pyridinium salt.

General Procedure for Reduction of Pyridinium Salt:

The pyridinium salt (crude) was dissolved in methanol and sodium borohydride (113 mg, 3 eq) was added. This solution was warmed to 35° C. for 5 hours then cooled to room temperature. The methanol was removed, water and methylene chloride added, and extracted. The 150 mg (70% yield) of tetrahydropyridine was produced after column chromatography.

General Procedure for Carbamate Formation:

The Compound D (31 mg, 0.074 mmol) was dissolved in methylene chloride (2 nil) at room temperature. Ethyl chloroformate (14 μl, 2 eq) was added to the solution and the reaction was stirred for 3 hours. The solvent was removed and the crude mixture was purified by flash chromatograph with ethyl acetate/hexane to produce 28.4 mg (97% yield) of Compound E.

General Procedure for Tetrahydropyridine Deprotection:

10% Palladium on carbon (10 mg, 10% w/w) was added to a methanolic solution (1.5 ml) of Compound F (109 mg, 0.247 mmol). A balloon of hydrogen was added to the top of the flask and thoroughly stirred for 1 hour. After filtration pure Compound G was recovered (73 mg, 96% yield). No further purification was required.

General Procedure for Thiazole Amine Formation:

Compound D (725 mg, 1.73 mmol) was dissolved in methylene chloride (5 ml) and cyanogen bromide (549 mg, 5.19 mmol) was added. This reaction was stirred at room temperature for 1 hour then purified by flash chromatography. Pure cyanamide (415 mg, 68% yield) was isolated as a white solid.

The cyanamide (55 mg, 0.01556 mmol) was dissolved in ethanol/ammonia (2 ml, 2M). Hydrogen sulfide gas was then bubbled through the reaction solution for 20 minutes at which time the bubbling was stopped, and the reaction was capped and allowed to stirred for 5 hours at room temperature. After completion, the solvent was removed and the thiourea was recrystallized from ethanol to remove yellow color.

The thiourea was then dissolved in anhydrous THF (5 ml) and the bromide (140 mg, 0.709 mmol) was added and refluxed for 5 hours. The reaction mixture was then quenched with sodium bicarbonate solution and diluted with ethyl acetate. After drying over magnesium sulfate, the compound was purified by flash chromotagraphy to yield 39 mg of pure Compound H. The hydrochloride salt of Compound H was prepared by dissolving

Compound H in ethanol and excess HCl in ether was added. Solid precipitated out of solution at −40° C. and was collected by filtration to yield the hydrochloride salt.

General Procedure for Palladium Amine Coupling:

Compound J (200 mg, 0.642 mmol) was dissolved in toluene/MeOH/H₂O (6 ml/0.5 ml/1 ml) followed by 2-bromo-pyridine (101 mg 0.77 mmol), Pd (20 mg, 10% w/w), heated to 170° C. for 1 hour. The solvent was removed and it was dissolved in methylene chloride and purified by flash column to give Compound K (20 mg, 8% yield).

Example 5 N-[4-(1-pyridin-2-yl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-3-fluoro-isonicotinamide

Procedure: General Procedure for Palladium Amine Coupling.

NMR (300 MHz, CDCl₃) δ8.67 (d, J=2.7 Hz, 1H), 8.65 (dd, J=1.2, 4.8 Hz, 1H), 8.40 (d, J=13.8 Hz, 1H), 8.21 (ddd, J=0.9, 1.8, 4.8 Hz, 1H), 8.04 (dd, J=4.8, 6.6 Hz, 1H), 7.68-7.62 (m, 2H), 7.51-7.45 (m, 1H), 7.32-7.26 (m, 1H), 6.66-6.58 (m, 2H), 4.09-4.06 (m, 2H), 3.83 (t, J=5.7 Hz, 2H), 2.33 (s, 2H), 1.67 (s, 3H) ppm

ESMS calcd. (C₂₃H₂₁FN₄O): 388.2; found: 389.2 (M+H).

Example 6 Ethyl 3-(6-(2,6-difluorobenzamido)pyridin-3-yl)-4-methyl-5,6-dihydropyridine-1(2H)-carboxylate

ESMS calcd. (C₂₁H₂₁F₂N₃O₃): 401.2; found: 402.3 (M+H).

Procedure:

See general procedure for Carbamate Formation, beginning with N-(5-(1-benzyl-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyridin-2-yl)-2,6-difluorobenzamide.

Example 7 2,6-difluoro-N-(5-(4-methyl-1-(pyrimidin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide hydrochloride

Pyrimidyl Amine Synthesis:

Procedure:

The tetrahydropyridine (25 mg, 0.076 mmol), 2-chloropyrimidine (17.3 mg, 0.152 mmol), and diisopropylamine (53 μl, 0.303 mmol) was dissolved in 5 ml dichloromethane in a microwave tube. It was heated to 135° C. for 1 hour. The methylene chloride solution was then loaded on a column. Although the compound was not completely pure, when HCl in ether was added the pure salt fell out of the ethyl acetate solution. 4 mg was isolated in a 13% yield.

ESMS calcd. (C₂₁H₁₉ClF₂N₆O): 444.1; found: 408.2 (M-Cl).

Example 8 Allyl 4-(4-(2,6-difluorobenzamido)phenyl)-3-methyl-5,6-dihydropyridine-1(2H)-carboxylate

ESMS calcd. (C₂₃H₂₂F₂N₂O₃): 412.2; found: 413.2 (M+H).

Procedure:

Synthesized using the general thiazole procedure and using allyl chloroformate.

Example 9 2,6-difluoro-N-(5-(4-methyl-1-picolinoyl-1,2,5,6-tetrahydropyridin-3-yl)pyridin-2-yl)benzamide hydrochloride

ESMS calcd. (C₂₄H₂₁ClF₂N₄O₂): 470.1; found: 435.2 (M-Cl).

Procedure:

See Example of Substitution of amine with Acid chloride/sulfonyl chloride beginning with 2,6-difluoro-N-(5-(4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyridin-2-yl)benzamide and picolinoyl chloride followed by acid formation using procedure (free base dissolved in ethyl acetate and 2 eq. of HCl in diethyl ether added, the ppt was filtered and dried).

Example 10 Ethyl 3-(acetoxymethyl)-4-(4-(2,6-difluorobenzamido)phenyl)-5,6-dihydropyridine-1(2H)-carboxylate

Procedure

Ethyl 1-benzyl-4-(4-(2,6-difluorobenzamido)phenyl)-1,2,5,6-tetrahydropyridine-3-carboxylate was made in the same fashion as the first step of thiazole amin formation. (200 mg, 0.42 mmol) was dissolved in 5 ml of THF. Lithium aluminum hydride (48 mg, 1.26 mmol) was added as a solid at room temperature and the reaction was stirred for 20 minutes. It was then quenched with water and stirred until the solid was white. After drying with magnesium sulfate the allylic alcohol was dissolved in methylene chloride (5 mls). Pyridine (100 mg, 1.26 mmol) and acetic anhydride (128.5 mg, 1.26 mmol) were added to the reaction and it was stirred overnight. Quenched with sodium bicarbonate(sat) solution and purified by column chromatography. 50 mg of pure acetate was isolated.

(1-benzyl-4-(4-(2,6-difluorobenzamido)phenyl)-1,2,5,6-tetrahydropyridin-3-yl)methyl acetate (15 mg, 0.031 mmol) was then dissolved in methylene chloride (5 ml) and ethyl chloroformate was added. This reaction was warmed to 40° C. for 30 minutes. Quenched with sodium bicarbonate solution and purified by flash chromatography. 5 mg (36% yield) was isolated

ESMS calcd. (C₂₄H₂₄F₂N₂O₅): 458.2; found: 459.2 (M+H).

Example 11 ethyl 4-chloro-3-(4-(2,6-difluorobenzamido)phenyl)-5,6-dihydropyridine-1(2H)-carboxylate

ESMS calcd. (C₂₁H₁₉ClF₂N₂O₃): 420.1; found: 421.1 (M+H).

Procedure:

See general procedure for carbamate formation.

Example 12 2,6-difluoro-N-(5-(4-methyl-1-(pyrazin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide (Compound II)

Procedure:

The amine (2 g, 6.2 mmol) was dissolved in dichloromethane (0.1M) and cooled to 0° C. The allyl chloroformate (1.32 ml, 12.4 mmol) was added and stirred for 45 min. Quenched with sat. sodium bicarbonated solution and the dichoromethane layer was rotovaped onto silica gel. A quick column afforded clean carbamate and removed benzyl chloride. This solid was dissolved in THF and 5 ml of pyrollidine was added along with 10% w/w palladium tetrakis and THF. Heated to reflux for 3 hrs. Purified by column on alumina with dichloromethane/methanol. Isolated 1.09 g (76% yield) of pure product.

General Aryl Amine Synthesis Using KF:

The amine, KF and pyrizine were added to DMSO solution and heated in microwave for 15 min at 100° C. After cooling, water was added to the DMSO solution followed by dichoromethane. The organic layer as separated and evaporated. Isolated pure product was obtained via column chromatography.

General Procedure for Pyrazine Amide Exchange:

The acetamide was dissolved in dichloromethane and diisopropyl ethylamine was added at room temperature. The acid chloride was then added and stirred for 2 hours. Quenched with saturated solution of sodium bicarbonate. The organic layer was separated and the dichloromethane was removed. The residue was dissolved in Methanol and potassium carbonate was then added followed by heating to reflux for 1 min, then evaporated onto silica gel and purifed by flash chromatography.

¹H NMR (300 MHz, CDCl₃) 9.70 (s, 1H), 8.46 (s, 1H), 8.27 (d, J=1.2 Hz, 1H), 8.19 (d, J=1.2 Hz, 1H), 8.08-8.09 (m, 1H), 7.84 (d, J=1.8 Hz, 1H), 7.45-7.53 (m, 1H), 7.05 (t, J=6 Hz, 2H), 4.37-4.39 (m, 2H), 3.88 (t, J=4.5 Hz, 2H), 2.39-2.41 (m, 2H), 1.86 (s, 3H) ppm ESMS calcd. (C₂₁H₁₈F₂N₆O) 408.2; found: 409.2 (M+H).

Example 13 2,6-difluoro-N-(5-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyridin-2-yl)benzamide (Compound 7)

¹H NMR (300 MHz, CDCl₃) δ 9.01 (s, 1H), 8.37 (d, J=6.3 Hz 1H), 8.22 (d, J=2.7 Hz, 1H), 8.01 (s, 1H), 7.65 (dd, J=1.5, 6.3 Hz, 1H), 7.40-7.52 (m, 2H), 7.00 (t, J=6.0 Hz, 2H), 6.60-6.66 (m, 2H), 4.06 (s, 2H), 3.81 (t, J=4.3 Hz, 2H) 2.34 (s, 2H), 1.66 (s, 3H) ppm. ESMS calcd. (C₂₃H₂₀F₂N₄O) 406.2; found: 407.2 (M+H).

Procedure.

The amine (179 mg, 0.544 mmol) was dissolved in a solution of acetonitrile (5 ml) and triethylamine (1 ml) was added. At room temperature, the benzyl pyridinium salt (154 mg, 0.544 mmol) was added and stirred for 1.5 hr. The solvent was evaporated and methanol was added along with 5% Pd/C (15 mg) and a hydrogen balloon was added to the top. After stirring overnight the solvent was removed and the product purified by column chromotagraphy using ethyl acetate hexane. The desired product was obtained as white solid (18 mg).

Example 14 2,6-difluoro-N-(5-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyridin-2-yl)benzamide hydrochloride (Compound 8)

A solution of the pyridine amine (8 mg) was made in methylene chloride and methanol. Excess HCl in ether was added and the solvent was then evaporated. The HCl salt was obtained as yellow solid (9 mg).

ESMS calcd. (C₂₃H₂₁ClF₂N₄O) 442.1; found: 407.2 (M-Cl).

Example 15 N-(5-(1-(3-chloropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide (Compound 12)

ESMS calcd. (C₂₁H₁₇ClF₂N₆O) 442.1; found: 443.2 (M+H).

See procedure for 2,6-difluoro-N-(5-(4-methyl-1-(pyrazin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide.

Example 16 2,6-difluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide (Compound 9)

ESMS calcd. (C₂₄H₂₁F₂N₃O) 405.2; found: 406.2 (M+H).

Procedure:

The amine (200 mg, 0.916 mmol) was dissolved in a solution of acetonitrile (5 ml) and triethylamine (1 ml) was added. At room temperature, the benzyl pyridinium salt (188 mg, 0.916 mmol) was added and stirred for 1 hr. The solvent was evaporated and methanol was added along with 10% Pd/C (20 mg) and a hydrogen balloon was added to the top. After stirring 4 hour the solvent was removed and the product purified by column chromotagraphy using a mixture of ethyl acetate and hexane. The aryl amine intermediate was obtained as a syruo (187 mg). The amine was then coupled with a carboxylic acid using general EDC coupling procedure to form amide.

Example 17 2,4-difluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide (Compound 10)

ESMS calcd. (C₂₄H₂₁F₂N₃O) 405.2; found: 406.2 (M+1).

See synthesis of 2,6-difluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide

Example 18 2,6-difluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide hydrochloride (Compound 13)

ESMS calcd. (C₂₃H₂₁C₁₃N₄O) 474.08; found: 439.1 (M-Cl).

2,6-difluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide hydrochloride

Example 19 2-chloro-6-fluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide hydrochloride (Compound 14)

ESMS calcd. (C₂₄H₂₂C₁₂FN₃O) 457.1; found: 422.1 (M-Cl)

2-chloro-6-fluoro-N-(4-(4-methyl-1-(pyridin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide hydrochloride

Example 20 N-(5-(1-(3-chloropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide hydrochloride (Compound 15)

¹H NMR (300 MHz, CDCl₃) 9.53 (s, 1H), 8.45 (s, 1H), 8.35 (dd, J=0.9, 5.7 Hz, 1H), 8.13 (dd, J=0.9, 4.5, 6.9, Hz, 1 Hz), 7.53-7.61 (m, 1H), 7.26 (dd, J=4.5, 5.7 Hz, 1H), 7.13 (t, J=6 Hz, 2H), 4.52 (s, 2H), 3.98 (t, J=4.2 Hz, 2H), 2.63-2.64 (m, 2H), 1.95 (s, 3H) ppm ESMS calcd. (C₂₂H₁₉Cl₂F₂N₅O) 477.1; found: 442.1 (M-Cl).

Example 21 2N-(5-(1-(3-chloropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,4-difluorobenzamide (Compound 16)

¹H NMR (300 MHz, CDCl₃) 9.67 (d, J=0.9 Hz, 1H), 8.95 (d, J=10.8 Hz, 1H), 8.31 (d, J=0.9 Hz, 1H), 8.25 (dd, J=4.8, 6.9, 13.5 Hz, 1 Hz), 8.09 (d, J=1.8 Hz, 1H), 7.85 (d, J=1.8 Hz, 1H), 7.07-7.12 (m, 1H), 6.96-7.01 (m, 1H), 4.32-4.34 (m, 2H), 3.74 (t, J=4.2 Hz, 2H), 2.48-2.49 (m, 2H), 1.86 (s, 3H) ppm ESMS calcd. (C₂₁H₁₇ClF₂N₆O) 442.1; found: 443.2 (M+H).

Example 22 22,6-difluoro-N-(5-(1-(3-fluoropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide hydrochloride (Compound 17)

¹H NMR (300 MHz, DMSO d6) 11.70 (s, 1H), 9.42 (s, 1H), 8.47 (s, 1H), 8.02 (d, J=3.6 Hz, 1H), 7.54-7.63 (m 2H), 7.26 (t, J=6 Hz, 2H), 6.80-6.90 (m, 1H), 4.31 (s, 2H), 3.60-3.70 (m, 2H), 2.38-2.39 (m, 2H), 1.83 (s, 3H) ppm. ESMS calcd. (C₂₂H₁₉ClF₃N₅O) 461.1; found: 425.2 (M-Cl)

Example 23 2,4-difluoro-N-(5-(1-(3-fluoropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide hydrochloride (Compound 18)

ESMS calcd. (C₂₂H₁₉ClF₃N₅O) 461.1; found: 425.2 (M-Cl)

See procedure for 2,6-difluoro-N-(5-(4-methyl-1-(pyrazin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide.

Example 24 N-(5-(1-(3-cyanopyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide (Compound 19)

⁽¹H NMR (300 MHz, CDCl₃) 9.68 (s, 1H), 8.27 (s, 1H), 7.34 (dd, J=1.5, 3.6 Hz, 1H), 8.16-8.17 (m, 1H), 7.78 (dd, J=1.5, 5.7 Hz, 1H), 7.43-7.50 (m, 1H), 7.03 (t, J=6.0 Hz, 2H), 6.73 (dd, J=3.6, 5.7 Hz, 1H), 4.49-4.50 (m, 2H), 3.97 (t, J=4.2 Hz, 2H), 2.49-2.50 (m, 2H), 1.82 (s, 3H), ppm ESMS calcd. (C₂₃H₁₈F₂N₆O) 432.2; found: 433.2 (M+H)

See procedure for 2,6-difluoro-N-(5-(4-methyl-1-(pyrazin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide

Example 25 N-(5-(1-(3,5-difluoropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide (Compound 20)

¹H NMR (300 MHz, CDCl₃) 9.67 (s, 1H), 8.45 (s, 1H), 8.23 (d, J=1.2 Hz, 1H), 7.94 (d, J=1.8 Hz, 1H), 7.45-7.52 (m, 1H), 7.13 (ddd, J=1.8, 5.7, 9.0 Hz, 1H), 7.04 (t, J=6.0 Hz, 2H), 4.25-4.26 (m, 2H), 3.35 (t, J=4.2 Hz, 2H), 2.41-2.50 (m, 2H), 1.82 (s, 3H), ppm ESMS calcd. (C₂₂H₁₇F₄N₅O) 443.1; found: 474.2 (M+H)

Example 26 N-(5-(1-(3,5-dichloropyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide (Compound 21)

⁽¹H NMR (300 MHz, CDCl₃) 9.66 (s, 1H), 8.48 (s, 1H), 8.22 (d, J=1.2 Hz, 1H), 8.11 (d, J=1.8 Hz, 1H), 7.60 (d, J=1.8 Hz, 1H), 7.44-7.51 (m, 1H), 7.04 (t, J=6.3 Hz, 2H), 4.20-4.21 (m, 2H), 3.59 (t, J=4.2 Hz, 2H), 2.45-2.46 (m, 2H), 1.83 (s, 3H), ppm ESMS calcd. (C₂₂H₁₇Cl₂F₂N₅O) 475.1; found: 476.2 (M+H)

Example 27 N-(5-(1-(3-bromopyridin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)-2,6-difluorobenzamide (Compound 22)

¹H NMR (300 MHz, CDCl₃-MeOD) 9.68 (s, 1H), 8.41-8.44 (m, 2H), 8.36 (d, J=0.9 Hz, 1H), 8.22 (dd, J=0.9, 5.7 Hz, 1H), 7.46-7.53 (m, 1H), 7.06 (t, J=6.3 Hz, 2H), 6.99 (dd, J=4.5, 5.7 Hz, 1H), 4.78 (s, 2H), 4.07 (t, J=4.5 Hz, 2H), 2.70-2.75 (m, 2H), 1.91 (s, 3H) ppm ESMS calcd. (C₂₂H₁₈BrF₂N₅O) 485.1; found: 486.2 (M+H) See procedure for 2,6-difluoro-N-(5-(4-methyl-1-(pyrazin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)pyrazin-2-yl)benzamide

Example 28 N-(4-(1-(3-aminopyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)phenyl)-2,6-difluorobenzamide (Compound 23)

¹H NMR (400 MHz, CDCl₃) δ (ppm), 8.32-6.80 (m, 9H), 4.63 (s, 2H), 3.39 (m, 2H), 2.35 (m, 2H), 1.46 (s, 3H); ESMS clcd for C₂₃H₂₁F₂N₅O: 421.2; Found: 422.2 (M+H)⁺.

Example 29 4-(1-(3-chloropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)-N-(2,6-difluorobenzyl)aniline (Compound 24)

¹H NMR (400 MHz, CDCl₃) δ (ppm), 8.03 (s, 1H), 7.78 (s, 1H), 7.32-6.60 (m, 7H), 4.33 (s, 2H), 4.05 (s, 2H), 3.69 (m, 2H), 2.36 (m, 2H), 1.55 (s, 3H); ESMS clcd for C₂₃H₂₁ClF₂N₄O: 426.1; Found: 427.1 (M+H)⁺.

Example 30 N-(4-(1-(3-cyanopyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)phenyl)-2,6-difluorobenzamide (Compound 25)

¹H NMR (400 MHz, CDCl₃) δ (ppm), 8.36-6.71 (m, 9H), 4.36 (s, 2H), 3.04 (m, 2H), 2.41 (s, 2H), 1.63 (s, 3H); ESMS clcd for C₂₄H₁₉F₂N₅O: 431.2; Found: 432.2 (M+H)⁺.

Example 31 2,6-difluoro-N-(4-(1-(3-fluoropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide (Compound 26)

¹H NMR (400 MHz, CDCl₃) δ (ppm), 7.91-6.82 (m, 9H), 4.23 (s, 2H), 3.70 (m, 2H), 2.34 (s, 2H), 1.65 (s, 3H); ESMS clcd for C₂₃H₁₉F₃N₄O: 424.2; Found: 425.2 (M+H)⁺.

Example 32 N-(4-(1-(3-chloropyrazin-2-yl)-4-methyl-1,2,5,6-tetrahydropyridin-3-yl)phenyl)-2,6-difluorobenzamide (Compound 27)

¹H NMR (400 MHz, CD₃OD) δ (ppm), 8.24-7.02 (m, 9H), 4.14 (s, 2H), 3.63 (m, 2H), 2.41 (s, 2H), 1.63 (s, 3H); ESMS clcd for C₂₃H₁₉ClF₂N₄O: 440.1; Found: 441.1 (M+H)⁺.

Example 33 2,6-difluoro-N-(4-(4-methyl-1-(pyrazin-2-yl)-1,2,5,6-tetrahydropyridin-3-yl)phenyl)benzamide (Compound 28)

¹H NMR (400 MHz, CD₃OD) δ (ppm), 8.34-6.97 (m, 9H), 4.22 (s, 2H), 3.81 (m, 2H), 2.41 (s, 2H), 1.65 (s, 3H); ESMS clcd for C₂₃H₂₀F₂N₄O: 406.2; Found: 407.2 (M+H)⁺.

Example A Inhibition of IL-2 Production

Jurkat cells were placed in a 96 well plate (0.5 million cells per well in 1% FBS medium) then a test compound of this invention was added at different concentrations. After 10 minutes, the cells were activated with PHA (final concentration 2.5 μg/mL) and incubated for 20 hours at 37° C. under CO₂. The final volume was 200 μL. Following incubation, the cells were centrifuged and the supernatants collected and stored at −70° C. prior to assaying for IL-2 production. A commercial ELISA kit (IL-2 Eli-pair, Diaclone Research, Besancon, France) was used to detect production of IL-2, from which dose response curves were obtained. The IC₅₀ value was calculated as the concentration at which 50% of maximum IL-2 production after stimulation was inhibited versus a non-stimulation control.

Compound # IC₅₀ (nM) 1 30 2 38 3 >1000 4 934 5 >1000 6 66

Inhibition of other cytokines, such as IL-4, IL-5, IL-13, GM-CSF, TNF-α, and INF-γ, can be tested in a similar manner using a commercially available ELISA kit for each cytokine.

Example B Patch Clamp Studies of Inhibition of I_(CRAC) Current in RBL Cells, Jurkat Cells, and Primary T Cells

In general, a whole cell patch clamp method was used to examine the effects of a compound of the invention on a channel that mediates I_(CRAC). In such experiments, a baseline measurement was established for a patched cell. Then a compound to be tested was perfused (or puffed) to cells in the external solution and the effect of the compound on I_(CRAC) was measured. A compound that modulates I_(CRAC) (e.g., inhibits) is a compound that is useful in the invention for modulating CRAC ion channel activity.

1) RBL Cells

Cells

Rat basophilic leukemia cells (RBL-2H3) are grown in DMEM media supplemented with 10% fetal bovine serum in an atmosphere of 95% air/5% CO₂. Cells are seeded on glass coverslips 1-3 days before use.

Recording Conditions

Membrane currents of individual cells are recorded using the whole-cell configuration of the patch clamp technique with an EPC10 (HEKA Electronik, Lambrecht, Germany). Electrodes (2-5 MΩ in resistance) are fashioned from borosilicate glass capillary tubes (Sutter Instruments, Novato, Ca). The recordings are done at room temperature.

Intracellular Pipette Solution

The intracellular pipette solution contains Cs-Glutamate 120 mM; CsCl 20 mM; CsBAPTA 10 mM; CsHEPES 10 mM; NaCl 8 mM; MgCl₂ 1 mM; IP3 0.02 mM; pH=7.4 adjusted with CsOH. The solution is kept on ice and shielded from light before the experiment is preformed.

Extracellular Solution

The extracellular solution contains NaCl 138 mM; NaHEPES, 10 mM; CsCl 10 mM; CaCl₂ 10 mM; Glucose 5.5 mM; KCl 5.4 mM; KH₂PO₄ 0.4 mM; Na₂HPO₄.H₂O 0.3 mM at pH=7.4 adjusted with NaOH.

Compound Treatment

Each test compound is diluted from a 10 mM stock in series using DMSO. The final DMSO concentration is kept at 0.1%.

Experimental Procedure

I_(CRAC) currents are monitored every 2 seconds using a 50 msec protocol, where the voltage was ramped from −100 mV to +100 mV. The membrane potential is held at 0 mV between the test ramps. In a typical experiment, the peak inward currents are expected to develop within 50-100 seconds. Once the I_(CRAC) currents are stabilized, the cells are perfused with a test compound in the extracellular solution. At the end of an experiment, the remaining I_(CRAC) currents are then challenged with a control compound (SKF96365, 10 μM) to ensure that the current could still be inhibited.

Data Analysis

The I_(CRAC) current level is determined by measuring the inward current amplitude at −80 mV of the voltage ramp in an off-line analysis using MATLAB. The I_(CRAC) current inhibition for each concentration is calculated using peak amplitude in the beginning of the experiment from the same cell. The IC₅₀ value and Hill coefficient for each compound is estimated by fitting all the individual data points to a single Hill equation.

Results

Compounds of the invention are expected to inhibit I_(CRAC) current in RBL cells.

2) Jurkat Cells

Cells

Jurkat T cells are grown on glass coverslips, transferred to the recording chamber and kept in a standard modified Ringer's solution of the following composition: NaCl 145 mM, KCl 2.8 mM, CsCl 10 mM, CaCl₂ 10 mM, MgCl₂ 2 mM, glucose 10 mM, HEPES-NaOH 10 mM, pH 7.2.

Extracellular Solution

The external solution contains 10 mM CaNaR, 11.5 mM glucose and a test compound at various concentrations.

Intracellular Pipette Solution

The standard intracellular pipette solution contains: Cs-glutamate 145 mM, NaCl 8 mM, MgCl₂ 1 mM, ATP 0.5 mM, GTP 0.3 mM, pH 7.2 adjusted with CsOH. The solution is supplemented with a mixture of 10 mM Cs-BAPTA and 4.3-5.3 mM CaCl₂ to buffer [Ca²⁺]i to resting levels of 100-150 nM.

Patch-Clamp Recordings

Patch-clamp experiments are performed in the tight-seal whole-cell configuration at 21-25° C. High-resolution current recordings are acquired by a computer-based patch-clamp amplifier system (EPC-9, HEKA, Lambrecht, Germany). Sylgard®-coated patch pipettes typically have resistances between 2-4 MΩ after filling with the standard intracellular solution. Immediately following establishment of the whole-cell configuration, voltage ramps of 50 ms duration spanning the voltage range of −100 to +100 mV are delivered from a holding potential of 0 mV at a rate of 0.5 Hz over a period of 300 to 400 seconds. All voltages are corrected for a liquid junction potential of 10 mV between external and internal solutions. Currents are filtered at 2.3 kHz and digitized at 100 μs intervals. Capacitive currents and series resistance are determined and corrected before each voltage ramp using the automatic capacitance compensation of the EPC-9.

Data Analysis

The very first ramps before activation of I_(CRAC) (usually 1 to 3) are digitally filtered at 2 kHz, pooled and used for leak-subtraction of all subsequent current records. The low-resolution temporal development of inward currents is extracted from the leak-corrected individual ramp current records by measuring the current amplitude at −80 mV or a voltage of choice.

Compounds of the invention are expected to inhibit I_(CRAC) current in Jurkat cells.

3) Primary T Cells

Preparation of Primary T Cells

Primary T cells are obtained from human whole blood samples by adding 100 μL of RosetteSep® human T cell enrichment cocktail to 2 mL of whole blood. The mixture is incubated for 20 minutes at room temperature, then diluted with an equal volume of PBS containing 2% FBS. The mixture is layered on top of RosetteSep® DM-L density medium and then centrifuged for 20 minutes at 1200 g at room temperature. The enriched T cells are recovered from the plasma/density medium interface, then washed with PBS containing 2% FBS twice, and used in patch clamp experiments following the procedure described for RBL cells.

Compounds of the invention are expected to inhibit I_(CRAC) current in human primary T cells.

Example C Inhibition of Multiple Cytokines in Primary Human PBMCs

Peripheral blood mononuclear cells (PBMCs) are stimulated with phytohemagglutinin (PHA) in the presence of varying concentrations of compounds of the invention or cyclosporine A (CsA), a known inhibitor of cytokine production. Cytokine production is measured using commercially available human ELISA assay kits (from Cell Science, Inc.) following the manufacturers instructions.

The compounds of the invention are potent inhibitors of IL-2, and are expected to be potent inhibitors of IL-4, IL-5, IL-13, GM-CSF, INF-α and TNF-γ in primary human PBM cells. In addition, compounds of the invention are not expected to inhibit the anti-inflammatory cytokine, IL-10.

Example D Compounds of the Invention are Potent Inhibitors of Degranulation in RBL Cells

Procedure:

The day before the assay is performed, RBL cells, that had been grown to confluence in a 96 well plate, are incubated at 37° C. for at least 2 hours. The medium is replaced in each well with 100 μL of fresh medium containing 2 μLg/mL of anti-DNP IgE.

On the following day, the cells are washed once with PRS (2.6 mM glucose and 0.1% BSA) and 160 μL of PRS was added to each well. A test compound is added to a well in a 20 μL solution at 10× of the desired concentration and incubated for 20 to 40 minutes at 37° C. 20 μL of 10× mouse anti-IgE (10 μL/mL) is added. SKF96365 is used as a positive control. Maximum degranulation typically occurs between 15 to 40 minutes after addition of anti-IgE.

Results:

Compounds of the invention are expected to inhibit degranulation of RBL cells.

Example E Compounds of the Invention are Potent Inhibitors of Chemotaxis in T Cells

T-Cell Isolation:

Twenty ml aliquots of heparinized whole blood (2 pig, 1 human) are subjected to density gradient centrifugation on Ficoll Hypaque. The buffy coat layers representing peripheral blood mononuclear cells (PBMCs) containing lymphocytes and monocytes are washed once, resuspended in 12 ml of incomplete RPMI 1640 and then placed in gelatin-coated T75 culture flasks for 1 hr at 37° C. The non-adherent cells, representing peripheral blood lymphocytes (PBLs) depleted of monocytes, are resuspended in complete RPMI media and placed in loosely packed activated nylon wool columns that had been equilibrated with warm media. After 1 hr at 37° C., the non-adherent T cell populations are eluted by washing of the columns with additional media. The T cell preparations are centrifuged, resuspended in 5 ml of incomplete RPMI, and counted using a hemocytometer.

Cell Migration Assay:

Aliquots of each T cell preparation are labeled with Calcien AM (TefLabs) and suspended at a concentration of 2.4×10⁶/ml in HEPES-buffered Hank's Balanced Salt Solution containing 1.83 mM CaCl₂ and 0.8 mM MgCl₂, pH 7.4 (HHBSS). An equal volume of HHBSS containing 0, 20 nM, 200 nM or 2000 nM of compound I or 20 nM EDTA is then added and the cells are incubated for 30 mM at 37° C. Fifty μl aliquots of the cell suspensions (60,000 cells) are placed on the membrane (pore size 5 μm) of a Neuroprobe ChemoTx 96 well chemotaxis unit that had been affixed over wells containing 10 ng/ml MIP-1α in HHBSS. The T cells are allowed to migrate for 2 hr at 37° C., after which the apical surface of the membrane is wiped clean of cells. The chemotaxis units are then placed in a CytoFlour 4000 (PerSeptive BioSystems) and the fluorescence of each well is measured (excitation and emission wavelengths of 450 and 530 nm, respectively). The number of migrating cells in each well is determined from a standard curve generated from measuring the fluorescence of serial two-fold dilutions of the labeled cells placed in the lower wells of the chemotaxis unit prior to affixing the membrane.

Results:

Compounds of the invention are expected to be inhibitory to the chemotactic response of porcine T cells and in human T cells.

All publications, patent applications, patents, and other documents cited herein are incorporated by reference in their entirety. In case of conflict, the present specification, including definitions, will control. In addition, the materials, methods, and examples are illustrative only and not intended to be limiting in any way.

From the foregoing description, it will be apparent that variations and modifications may be made to the invention described herein. Such embodiments are also within the scope of the following claims. 

1. A compound represented by the following structural formula:

or a pharmaceutically acceptable salt, solvate, clathrate, or prodrug thereof, wherein: X₁ and X₂ are, independently, CH or N; one of X₃ or X₆ is NR₂₉, and the other is CH₂, CHR₂, or C(R₂)₂; L is a linker selected from the group consisting of —NHCH₂—, —CH₂NH—, —NR₅—C(O)—, —C(O)—NR₅—, —NR₅—C(S)—, —C(S)—NR₅—, —NR₅—C(NR₈)—, —C(NR₈)—NR₅—, —NR₅C(S)NR₅—, —NR₅C(NR₈)NR₅—, and —NR₅CR^(a)R^(b)NR₅—; Y is a substituted phenyl, provided that Y is not substituted with —NHC(O)R₃₀; R₁ is H or a substituent; R₂, for each occurrence is, independently, a substituent, provided that R₂ is not ═O or ═C(R₂₈)₂; R₄ is a substituent; R^(a) and R^(b), for each occurrence, are independently, H, an optionally substituted alkyl, an optionally substituted alkenyl, an optionally substituted alkynyl, an optionally substituted cycloalkyl, an optionally substituted cycloalkenyl, an optionally substituted heterocyclyl, an optionally substituted aryl, an optionally substituted heteroaryl, an optionally substituted aralkyl, an optionally substituted heteraralkyl, cyano, nitro, halo, —OR₅, —SR_(S), —NR₆R₇, —C(O)NR₆R₇, —NR₅C(O)R₅, —C(O)R₅, —C(O)OR₅, —OC(O)R₅, —C(O)SR₅, —SC(O)R₅, —C(S)NR₆R₇, —NR₅C(S)R₅, —C(S)R₅, —C(S)OR₅, —OC(S)R₅, —C(S)SR₅, —SC(S)R₅, —C(NR₈)NR₆R₇, —NR₅C(NR₈)R₅, —C(NR₈)R₅, —C(NR₈)OR₅, —OC(NR₈)R₅, —C(NR₈)SR₅, —SC(NR₈)R₅, —OC(O)OR₅, —OC(O)NR₆R₇, —NR₅C(O)OR₅, —NR₅C(O)NR₆R₇, —SC(O)OR₅, —SC(O)NR₆R₇, —SC(O)SR₅, —NR₅C(O)SR₅, —OC(O)SR₅, —OC(S)OR₅, —OC(S)NR₆R₇, —NR₅C(S)OR₅, —NR₅C(S)NR₆R₇, —SC(S)OR₅, —SC(S)NR₆R₇, —SC(S)SR₅, —NR₅C(S)SR₅, —OC(S)SR₅, —OC(NR₈)OR₅, —OC(NR₈)NR₆R₇, —NR₅C(NR₈)OR₅, —NR₅C(NR₈)NR₆R₇, —SC(NR₈)OR₅, —SC(NR₈)NR₆R₇, —SC(NR₈)SR₅, —NR₅C(NR₈)SR₅, or —OC(NR₈)SR₅; R₅, for each occurrence, is independently, H, an optionally substituted alkyl, an optionally substituted alkenyl, an optionally substituted alkynyl, an optionally substituted cycloalkyl, an optionally substituted cycloalkenyl, an optionally substituted heterocyclyl, an optionally substituted aryl, an optionally substituted heteroaryl, an optionally substituted aralkyl, or an optionally substituted heteraralkyl; R₆ and R₇, for each occurrence are, independently, H, an optionally substituted alkyl, an optionally substituted alkenyl, an optionally substituted alkynyl, an optionally substituted cycloalkyl, an optionally substituted cycloalkenyl, an optionally substituted heterocyclyl, an optionally substituted aryl, an optionally substituted heteroaryl, an optionally substituted aralkyl, or an optionally substituted heteraralkyl; or R₆ and R₇ taken together with the nitrogen to which they are attached are an optionally substituted heterocyclyl or optionally substituted heteroaryl; R₈, for each occurrence, is independently —H, a halo, an alkyl, —OR₅, —NR₆R₇, —C(O)R₅, —C(O)OR₅, or —C(O)NR₆R₇; R₂₈, for each occurrence is, independently, H or a substituent; R₂₉ is —C(O)R₃₀ or an optionally substituted N containing 6-membered aromatic ring; R₃₀ is —H, —OR₅, —SR_(S), —NR₆R₇, an optionally substituted alkyl, an optionally substituted alkenyl, an optionally substituted alkynyl, an optionally substituted cycloalkyl, an optionally substituted cycloalkenyl, an optionally substituted heterocyclyl, an optionally substituted aryl, an optionally substituted heteroaryl, an optionally substituted aralkyl, or an optionally substituted heteraralkyl; m is 0, 1, or 2; n is 0, 1 or 2; and with the proviso that the compound is not: 5-[4-(2,6-difluoro-benzoylamino)-phenyl]-4-methyl-2,4-dihydro-2H-pyridine-1-carboxylic acid ethyl ester; Ethyl 3-(4-(2,6-difluorobenzamido)phenyl)-4-methyl-5,6-dihydropyridine-1(2H)-carboxylate; Ethyl 4-(4-(2,6-difluorobenzamido)phenyl)-3-methyl-5,6-dihydropyridine-1(2H)-carboxylate; 2,6-Difluoro-N-[4-(1-methoxycarbonyl-4-thiazol-2-yl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; 2,6-Difluoro-N-[4-(1-methoxycarbonyl-4-isopropyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; 2,6-Difluoro-N-[4-(1-methoxycarbonyl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; 2,6-Difluoro-N-[4-(1-allyloxycarbonyl-3-methyl-1,2,5,6-tetrahydro-pyridin-4-yl)-phenyl]-benzamide; 2,6-Difluoro-N-[4-(1-phenyloxycarbonyl-3-methyl-1,2,5,6-tetrahydro-pyridin-4-yl)-phenyl]-benzamide; 2,6-Difluoro-N-[4-(1-acetyl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; 2,6-Difluoro-N-[4-(1-cyclopropylcarbonyl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide; 2,6-Difluoro-N-{4-[1-(pyridine-3-carbonyl)-cyclopropanecarbonyl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl]-phenyl}-benzamide; or 2,6-Difluoro-N-[4-(1-pyrimidin-2-yl-4-methyl-1,2,5,6-tetrahydro-pyridin-3-yl)-phenyl]-benzamide.
 2. The compound of claim 1, wherein Y is substituted with one to two substituents.
 3. The compound of claim 2, wherein the one to two substituents are each independently a lower alkyl or a halo.
 4. The compound of claim 3, wherein Y is a difluorophenyl.
 5. The compound of claim 4, wherein Y is 2,6-difluorophenyl.
 6. The compound of claim 1, wherein L is —NHCH₂—, —CH₂NH—, —NH—C(O)—, or —C(O)—NH—.
 7. The compound of claim 6, wherein L is —NHC(O)—.
 8. The compound of claim 1, wherein R₁ is selected from the group consisting of a halo, an optionally substituted alkyl, an optionally substituted alkenyl, an optionally substituted alkynyl, an optionally substituted cycloalkyl, an optionally substituted cycloalkenyl, an optionally substituted heterocyclyl, an optionally substituted aryl, an optionally substituted heteroaryl, an optionally substituted aralkyl, and an optionally substituted heteraralkyl.
 9. The compound of claim 8, wherein R₁ is selected from the group consisting of halo, and an optionally substituted lower alkyl.
 10. The compound of claim 1, wherein X₃ is CH₂ and X₆ is NR₂₉.
 11. The compound of claim 1, wherein X₆ is CH₂ and X₃ is NR₂₉.
 12. The compound of claim 1, wherein X₁ and X₂ are both CH or both N.
 13. (canceled)
 14. The compound of claim 1, wherein X₁ is N and X₂ is CH.
 15. (canceled)
 16. The compound of claim 1, wherein Y is a difluorophenyl; L is —NHC(O)—; R₁ is selected from the group consisting of halo and an optionally substituted lower alkyl; and m is
 0. 17. The compound of claim 1, wherein the compound is represented by one of the following structures:

wherein R₃₁ is halo, an optionally substituted alkyl, an optionally substituted alkenyl, an optionally substituted alkynyl, an optionally substituted cycloalkyl, an optionally substituted cycloalkenyl, an optionally substituted heterocyclyl, an optionally substituted aryl, an optionally substituted heteroaryl, an optionally substituted aralkyl, or an optionally substituted heteraralkyl; and p is 1, 2, 3, 4 or
 5. 18-30. (canceled)
 31. The compound of claim 17, wherein the compound is represented by one of the following structures:

32-47. (canceled)
 48. The compound of claim 1, wherein the compound is represented by one of the following structures:

wherein each of X₇, X₈, X₉, X₁₀, and X₁₁ are independently CH, CR₂, or N, provided that at least one of X₇, X₈, X₉, X₁₀, or X₁₁ is N; R₃₁ is halo, an optionally substituted alkyl, an optionally substituted alkenyl, an optionally substituted alkynyl, an optionally substituted cycloalkyl, an optionally substituted cycloalkenyl, an optionally substituted heterocyclyl, an optionally substituted aryl, an optionally substituted heteroaryl, an optionally substituted aralkyl, or an optionally substituted heteraralkyl; and p is 1, 2, 3, 4 or
 5. 49-59. (canceled)
 60. The compound of claim 48, wherein the compound is represented by one of the following structures:

61-76. (canceled)
 77. A pharmaceutical composition, comprising a pharmaceutically acceptable carrier and a compound of claim
 1. 78. The pharmaceutical composition of claim 77, further comprising one or more additional therapeutic agents. 79-106. (canceled) 